1887

Abstract

A novel filamentous bacterial strain, A-T 5190, which developed irregular sporangia at the end of sporangiophores on substrate mycelia, was isolated from dry evergreen forest soil collected in Thailand. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5190 belonged to the genus and was related most closely to NBRC 14916 (98.88 % similarity) and NBRC 13941 (98.54 %). DNA–DNA relatedness values between strain A-T 5190 and its closest relatives were below 70 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, galactose and xylose. The predominant menaquinone was MK-9(H). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acid C and branched fatty acids iso-C, iso-C and anteiso-C. The G+C content of the genomic DNA was 71.9 mol%. Evidence from phenotypic, chemotaxonomic and genotypic studies indicate that strain A-T 5190 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A-T 5190 ( = BCC 41582 = NBRC 109644).

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2015-11-01
2019-12-08
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