Strain THG-MD12T, a Gram-reaction-positive, aerobic, non-motile, rod-shaped bacterium was isolated from rhizosphere soil of Ophiopogon japonicus in PR China. THG-MD12T was closely related to members of the genus Pseudoclavibacter and showed the highest 16S rRNA gene sequence similarities with Pseudoclavibacter helvolus KCTC 19531T (98.8 %) and Pseudoclavibacter chungangensis KCTC 22691T (96.9 %). DNA–DNA hybridization showed 41.9 ± 2.1 % and 12.4 ± 0.9 % DNA reassociation with P. helvolus KCTC 19531T and P. chungangensis KCTC 22691T, respectively. Chemotaxonomic analyses revealed that strain THG-MD12T possesses menaquinone-9 as the predominant respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan and anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0 as the major fatty acids. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and two unknown lipids. These data corroborated the affiliation of THG-MD12T to the genus Pseudoclavibacter. Thus, the isolate represents a novel species, for which the name Pseudoclavibacter terrae sp. nov. is proposed, with THG-MD12T as the type strain ( = CCTCC AB 2015124T = KCTC 39562T).
CollinsM.D.,
JonesD.
( 1981;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev45316–354[PubMed].
EzakiT.,
HashimotoY.,
YabuuchiE.
( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39224–229 [View Article].
HallT.A.
( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser4195–98.
KumarS.,
NeiM.,
DudleyJ.,
TamuraK.
( 2008;). MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform9299–306 [View Article][PubMed].
LinY.C.,
UemoriK.,
de BrielD.A.,
ArunpairojanaV.,
YokotaA.
( 2004;). Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol541669–1676 [View Article][PubMed].
McPhersonD.C.,
PophamD.L.
( 2003;). Peptidoglycan synthesis in the absence of class A penicillin-binding proteins in Bacillus subtilis
. J Bacteriol1851423–1431 [View Article][PubMed].
MesbahM.,
PremachandranU.,
WhitmanW.B.
( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39159–167 [View Article].
MinnikinD.E.,
PatelP.V.,
AlshamaonyL.,
GoodfellowM.
( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol27104–117 [View Article].
MooreD.D.,
DowhanD.
( 1995;). Preparation and Analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by
AusubelF. W.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
New York:
Wiley;.
ParkM.H.,
TraiwanJ.,
JungM.Y.,
KimW.
( 2012;). Gulosibacter chungangensis sp. nov., an actinomycete isolated from a marine sediment, and emended description of the genus Gulosibacter. Int J Syst Evol Microbiol621055–1060 [View Article][PubMed].
StackebrandtE.,
GoebelB.M.
( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol44846–849. [CrossRef]