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Abstract

Strain THG-MD12, a Gram-reaction-positive, aerobic, non-motile, rod-shaped bacterium was isolated from rhizosphere soil of in PR China. THG-MD12 was closely related to members of the genus and showed the highest 16S rRNA gene sequence similarities with KCTC 19531 (98.8 %) and KCTC 22691 (96.9 %). DNA–DNA hybridization showed 41.9 ± 2.1 % and 12.4 ± 0.9 % DNA reassociation with KCTC 19531 and KCTC 22691, respectively. Chemotaxonomic analyses revealed that strain THG-MD12 possesses menaquinone-9 as the predominant respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan and anteiso-C, iso-C, C and anteiso-C as the major fatty acids. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and two unknown lipids. These data corroborated the affiliation of THG-MD12 to the genus . Thus, the isolate represents a novel species, for which the name sp. nov. is proposed, with THG-MD12 as the type strain ( = CCTCC AB 2015124 = KCTC 39562).

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2015-11-01
2021-03-09
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