gen. nov., sp. nov., isolated from deep seawater Free

Abstract

A taxonomic study was carried out on strain 22II1-22F33, which was isolated from deep seawater of the Atlantic Ocean. The bacterium was Gram-stain-negative, oxidase-positive and weakly catalase-positive, oval in shape without flagellum. Growth was observed at salinities of 0–12 % and at temperatures of 4–41 °C. The isolate was capable of hydrolysing aesculin and Tween 80 and reduction of nitrate to nitrite, but unable to hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II1-22F33 belongs to the family , with highest sequence similarity to AZO-C (96.5 %). The principal fatty acids (>10 %) were summed feature 8 (Cω7/ω6c) (73.8 %). The G+C content of the genomic DNA was 66.2 mol%. The respiratory quinone was Q-10 (100 %). Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), two unidentified aminolipids (ALs), six unidentified phospholipids (PLs) and one unidentified lipid (L) were present. The combined genotypic and phenotypic data show that strain 22II1-22F33 represents a novel species within a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 22II1-22F33 ( = LMG 27151 = MCCC 1A09326).

Funding
This study was supported by the:
  • COMRA program (Award No. DY125-15-R-01)
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2015-11-01
2024-03-28
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. ( 2002). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology New York: Wiley;.
    [Google Scholar]
  2. Cho J.C., Giovannoni S.J. ( 2006;). Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 56 855859 [View Article] [PubMed].
    [Google Scholar]
  3. Dong X., Cai M. ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);.
    [Google Scholar]
  4. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  5. Garrity G., Bell J., Lilburn T. ( 2006;). Family I. Rhodobacteraceae fam. nov. List of new names and new combinations previously effectively, but not validly, published – Validation List 107. Int J Syst Evol Microbiol 56 16. [CrossRef]
    [Google Scholar]
  6. Hameed A., Shahina M., Lin S.Y., Lai W.A., Hsu Y.H., Liu Y.C., Huang Y.M., Young C.C. ( 2013;). Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006. Int J Syst Evol Microbiol 63 45334539 [View Article] [PubMed].
    [Google Scholar]
  7. Hyun D.W., Kim M.S., Shin N.R., Kim J.Y., Kim P.S., Whon T.W., Yun J.H., Bae J.W. ( 2013;). Shimia haliotis sp. nov., a bacterium isolated from the gut of an abalone, Haliotis discus hannai . Int J Syst Evol Microbiol 63 42484253 [View Article] [PubMed].
    [Google Scholar]
  8. Kates M. ( 1986). Lipid Extraction Procedures. Techniques of Lipidology Amsterdam: Elsevier;.
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  10. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed].
    [Google Scholar]
  11. Lai Q., Yuan J., Gu L., Shao Z. ( 2009;). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59 12781281 [View Article] [PubMed].
    [Google Scholar]
  12. Lai Q., Li G., Liu X., Du Y., Sun F., Shao Z. ( 2015;). Pseudooceanicola atlanticus gen. nov. sp. nov., isolated from surface seawater of the Atlantic Ocean and reclassification of Oceanicola batsensis, Oceanicola marinus, Oceanicola nitratireducens, Oceanicola nanhaiensis, Oceanicola antarcticus and Oceanicola flagellatus, as Pseudooceanicola batsensis comb. nov., Pseudooceanicola marinus comb. nov., Pseudooceanicola nitratireducens comb. nov., Pseudooceanicola nanhaiensis comb. nov., Pseudooceanicola antarcticus comb. nov., and Pseudooceanicola flagellatus comb. nov. Antonie van Leeuwenhoek 107 10651074 [View Article] [PubMed].
    [Google Scholar]
  13. Liu C., Shao Z. ( 2005;). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55 11811186 [View Article] [PubMed].
    [Google Scholar]
  14. Lucena T., Ruvira M.A., Macián M.C., Pujalte M.J., Arahal D.R. ( 2013;). Description of Tropicibacter mediterraneus sp. nov. and Tropicibacter litoreus sp. nov. Syst Appl Microbiol 36 325329 [View Article] [PubMed].
    [Google Scholar]
  15. Rzhetsky A., Nei M. ( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35 367375 [View Article] [PubMed].
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  17. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  18. Shieh W.Y., Chen Y.-W., Chaw S.-M., Chiu H.-H. ( 2003;). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53 479484 [View Article] [PubMed].
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  20. Tindall B. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128130 [View Article].
    [Google Scholar]
  21. Tindall B. ( 1990b;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199202 [View Article].
    [Google Scholar]
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