1887

Abstract

A Gram-staining-negative, aerobic, non-motile, coccus-shaped bacterium, designated SAORIC-696, was isolated from deep-sea water at a depth of 1700 m in the western North Pacific Ocean. Optimal growth of strain SAORIC-696 was observed at 15 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Strain SAORIC-696 formed a robust phylogenetic clade with members of the genus The 16S rRNA gene sequence similarity showed that strain SAORIC-696 was most closely related to (98.0 % similarity) and (97.3 %). The DNA–DNA relatedness between SAORIC-696 and two species of the genus was only 27–42 %. The DNA G+C content of strain SAORIC-696 was 43.1 mol% and predominant cellular fatty acids were Cω9 (36.8 %), C (22.5 %) and C 3-OH and/or iso-C I (10.8 %). Strain SAORIC-696 contained MK-7 as the only respiratory quinone. On the basis of taxonomic data collected in this study, it was concluded that strain SAORIC-696 represents a novel species of the genus for which the name sp. nov. is proposed, with the type strain SAORIC-696 ( = NBRC 110692 = KCTC 42681).

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2015-11-01
2019-10-23
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References

  1. Cho J.-C., Giovannoni S.J.. ( 2003;). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53: 1031–1036 [CrossRef] [PubMed].
    [Google Scholar]
  2. Cho J.-C., Vergin K.L., Morris R.M., Giovannoni S.J.. ( 2004;). Lentisphaera araneosa gen. nov., sp. nov, a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae. Environ Microbiol 6: 611–621 [CrossRef] [PubMed].
    [Google Scholar]
  3. Choi A., Yang S.J., Rhee K.H., Cho J.-C.. ( 2013;). Lentisphaera marina sp. nov., and emended description of the genus Lentisphaera. Int J Syst Evol Microbiol 63: 1540–1544 [CrossRef] [PubMed].
    [Google Scholar]
  4. Collins M.D.. ( 1985;). Analysis of isoprenoid quinones. Methods Microbiol 18: 329–366 [CrossRef].
    [Google Scholar]
  5. Connon S.A., Giovannoni S.J.. ( 2002;). High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol 68: 3878–3885 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  7. Fitch W.M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 27: 401–410.
    [Google Scholar]
  8. Jukes T.H., Cantor C.R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–32. Edited by Munro H. N.. New York: Academic Press; [CrossRef].
    [Google Scholar]
  9. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner,A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32: 1363–1371 [CrossRef] [PubMed].
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W.B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  12. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  13. Pruesse E., Peplies J., Glöckner F.O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28: 1823–1829 [CrossRef] [PubMed].
    [Google Scholar]
  14. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  15. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  16. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  17. Thrash J.C., Cho J.-C., Vergin K.L., Morris R.M., Giovannoni S.J.. ( 2010;). Genome sequence of Lentisphaera araneosa HTCC2155T, the type species of the order Lentisphaerales in the phylum Lentisphaerae. J Bacteriol 192: 2938–2939 [CrossRef] [PubMed].
    [Google Scholar]
  18. Tindall B.J., Sikorski J., Smibert R.M., Krieg N.R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R.., 3rd edn.., Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  19. Waterbury W.B., Watson S.W., Valois F.W., Franks D.G.. ( 1986;). Biological and ecological characterization of the marine unicellular cyanobacterium Synechococcus. Can Bull Fish Aquat Sci 214: 71–120.
    [Google Scholar]
  20. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
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