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Abstract

A Gram-stain-positive, aerobic, motile and non-spore-forming actinobacterium, strain Y32, was isolated from a deep-sea sediment of the western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus . Strain Y32 shared highest 16S rRNA gene sequence similarity of 97.8 % with 1A-C, followed by TT02-04 (97.4 %), 2C6-43 (97.2 %), JLT1488 (97.2 %), YIM 004 (97.0 %) and CC-NMPT-T3 (97.0 %). The organism grew in the presence of 0–10 % (w/v) NaCl, at 4–40 °C and at pH 6–11, with optimal growth occurring at 30–35 °C, at pH 7 and in the presence of 3.5 % (w/v) NaCl. The polar lipid profile of strain Y32 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two phosphatidylinositol mannosides. Strain Y32 contained MK-8(H) and MK-7(H) as the major components of the menaquinone system, and anteiso-C, iso-C and iso-C as the predominant fatty acids. Galactose was detected as the cell-wall sugar. The G+C content of the DNA was 71.2 mol%. Based on the results of phenotypic, genotypic and phylogenetic analyses, it is considered that strain Y32 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y32 ( = LMG 28101 = CGMCC 1.12782 = JCM 19765 = MCCC 1A09955).

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2015-11-01
2024-03-28
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References

  1. Altenburger P., Kämpfer P., Schumann P., Vybiral D., Lubitz W., Busse H.J. ( 2002;). Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52 875881 [View Article] [PubMed].
    [Google Scholar]
  2. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed].
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  4. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  5. Felsenstein J. ( 1985;). Confidence limits on phylogenies – an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  6. Fu Y., Li Q., Liu K., Xu Y., Wang Y., Jiao N. ( 2012;). Oceanitalea nanhaiensis gen. nov., sp. nov., an actinobacterium isolated from seawater. Int J Syst Evol Microbiol 62 24902494 [View Article] [PubMed].
    [Google Scholar]
  7. Hamada M., Tamura T., Ishida Y., Suzuki K. ( 2009;). Georgenia thermotolerans sp. nov., an actinobacterium isolated from forest soil. Int J Syst Evol Microbiol 59 18751879 [View Article] [PubMed].
    [Google Scholar]
  8. Huss V.A., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 [View Article] [PubMed].
    [Google Scholar]
  9. Kämpfer P., Arun A.B., Busse H.J., Langer S., Young C.C., Chen W.M., Schumann P., Syed A.A., Rekha P.D. ( 2010;). Georgenia soli sp. nov., isolated from iron-ore-contaminated soil in India. Int J Syst Evol Microbiol 60 10271030 [View Article] [PubMed].
    [Google Scholar]
  10. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  11. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed].
    [Google Scholar]
  12. Lechevalier M.P., Lechevalier H.A. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no 6), pp. 227291. Edited by Dietz T. D. W. Arlington, VA: Society for Industrial Microbiology;.
    [Google Scholar]
  13. Li W.J., Xu P., Schumann P., Zhang Y.Q., Pukall R., Xu L.H., Stackebrandt E., Jiang C.L. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57 14241428 [View Article] [PubMed].
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid-chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  15. Minnikin D.E., Patel P.V., Alshamaony L., Goodfellow M. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27 104117 [View Article].
    [Google Scholar]
  16. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  17. Rzhetsky A., Nei M. ( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35 367375 [View Article] [PubMed].
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  19. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101., Newark, DE: MIDI Inc;.
    [Google Scholar]
  20. Schleifer K.H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  21. Srinivas A., Rahul K., Sasikala Ch., Subhash Y., Ramaprasad E.V., Ramana ChV. ( 2012;). Georgenia satyanarayanai sp. nov., an alkaliphilic and thermotolerant amylase-producing actinobacterium isolated from a soda lake. Int J Syst Evol Microbiol 62 24052409 [View Article] [PubMed].
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  23. Tang S.K., Wang Y., Lou K., Mao P.H., Xu L.H., Jiang C.L., Kim C.J., Li W.J. ( 2009;). Kocuria halotolerans sp. nov., an actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 59 13161320 [View Article] [PubMed].
    [Google Scholar]
  24. Tang S.K., Wang Y., Lee J.C., Lou K., Park D.J., Kim C.J., Li W.J. ( 2010;). Georgenia halophila sp. nov., a halophilic actinobacterium isolated from a salt lake. Int J Syst Evol Microbiol 60 13171321 [View Article] [PubMed].
    [Google Scholar]
  25. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad-hoc-committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  26. Woo S.G., Cui Y., Kang M.S., Jin L., Kim K.K., Lee S.T., Lee M., Park J. ( 2012;). Georgenia daeguensis sp. nov., isolated from 4-chlorophenol enrichment culture. Int J Syst Evol Microbiol 62 17031709 [View Article] [PubMed].
    [Google Scholar]
  27. Xu L.H., Li W.J., Liu Z.H., Jiang C.L. ( 2007;). Physiological and biochemical characteristics. . In Actinomycete Systematic—Principle, Methods and Practice, pp. 4053, Edited by Zhang Y.Q., Li W.J., Tian X.P. Beijing: Science Press;.
    [Google Scholar]
  28. You Z.Q., Li J., Qin S., Tian X.P., Wang F.Z., Zhang S. ( 2013;). Georgenia sediminis sp. nov., a moderately thermophilic actinobacterium isolated from sediment. Int J Syst Evol Microbiol 63 42434247 [View Article] [PubMed].
    [Google Scholar]
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