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Abstract

A Gram-stain-positive, aerobic, motile and non-spore-forming actinobacterium, strain Y32, was isolated from a deep-sea sediment of the western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus . Strain Y32 shared highest 16S rRNA gene sequence similarity of 97.8 % with 1A-C, followed by TT02-04 (97.4 %), 2C6-43 (97.2 %), JLT1488 (97.2 %), YIM 004 (97.0 %) and CC-NMPT-T3 (97.0 %). The organism grew in the presence of 0–10 % (w/v) NaCl, at 4–40 °C and at pH 6–11, with optimal growth occurring at 30–35 °C, at pH 7 and in the presence of 3.5 % (w/v) NaCl. The polar lipid profile of strain Y32 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two phosphatidylinositol mannosides. Strain Y32 contained MK-8(H) and MK-7(H) as the major components of the menaquinone system, and anteiso-C, iso-C and iso-C as the predominant fatty acids. Galactose was detected as the cell-wall sugar. The G+C content of the DNA was 71.2 mol%. Based on the results of phenotypic, genotypic and phylogenetic analyses, it is considered that strain Y32 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y32 ( = LMG 28101 = CGMCC 1.12782 = JCM 19765 = MCCC 1A09955).

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2015-11-01
2019-10-14
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