1887

Abstract

A novel actinomycete strain, SMC 256, which developed small, globose sporangia at the ends of long sporangiophores on aerial mycelium, was isolated from soil collected in a mountain forest of Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMC 256 belonged to the genus , and the closest phylogenetically related species were BCC 29373 (98.9 % 16S rRNA gene sequence similarity), ATCC 27102 (98.2 %), ATCC 25243 (97.9 %) and ATCC 25242 (97.4 %). The DNA–DNA relatedness values that distinguished strain SMC 256 from previously described members of the genus were significantly below 70 %. The G+C content of the genomic DNA was 71.8 mol%. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars consisted of rhamnose, ribose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H). Mycolic acids were not detected. The diagnostic phospholipids were hydroxyphosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, unidentified phosphoglycolipids, unidentified phospholipids and an unidentified lipid. The predominant cellular fatty acids were iso-C, C and C 10-methyl. Following the evidence of phenotypic, chemotaxonomic and genotypic studies, it is proposed that strain SMC 256 represents a novel species in the genus , namely sp. nov. The type strain is SMC 256 ( = BCC 72675 = NBRC 110610).

Funding
This study was supported by the:
  • Mahidol University
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2015-11-01
2021-10-28
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References

  1. Arai T. ( 1975). Culture Media for Actinomycetes Tokyo: Japanese Society for Actinomycetes;.
    [Google Scholar]
  2. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed].
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in micro-dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  4. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  5. Felsenstein J. ( 1983;). Parsimony in systematics: biological and statistical issues. Annu Rev Ecol Syst 14 313333 [View Article].
    [Google Scholar]
  6. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  7. Gordon R.E., Barnett D.A., Handerhan J.E., Pang C.H.-N. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24 5463 [View Article].
    [Google Scholar]
  8. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  9. Itoh T., Kudo T., Parenti F., Seino A. ( 1989;). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39 168173 [View Article].
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R.M. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42 9891005 [View Article].
    [Google Scholar]
  11. Kelly K.L. ( 1964). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors., Washington, DC: US Government Printing Office;.
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  13. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [View Article].
    [Google Scholar]
  14. Labeda D.P. ( 2012;). Genus IX. Kutzneria Stackebrandt, Kroppenstedt, Jahnke, Kemmerling and Gurtler 1994, 267VP . . In Bergey's Manual of Systematic Bacteriology vol. 5 , 2nd edn.., pp. 13711375. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B. NewYork: Springer;.
    [Google Scholar]
  15. Lechevalier M.P., Lechevalier H. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20 435443 [View Article].
    [Google Scholar]
  16. Lechevalier M.P., de Bièvre C., Lechevalier H. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5 249260 [View Article].
    [Google Scholar]
  17. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  18. Monciardini P., Sosio M., Cavaletti L., Chiocchini C., Donadio S. ( 2002;). New PCR primers for the selective amplification of 16S rDNA from different groups of actinomycetes. FEMS Microbiol Ecol 42 419429.
    [Google Scholar]
  19. Saito H., Miura K.I. ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72 619629 [View Article] [PubMed].
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425.
    [Google Scholar]
  21. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  22. Shirling E.B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313340 [View Article].
    [Google Scholar]
  23. Stackebrandt E., Kroppenstedt R.M., Jahnke K.-D., Kemmerling C., Gurtler H. ( 1994;). Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium . Int J Syst Bacteriol 44 265269 [View Article].
    [Google Scholar]
  24. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 226231.
    [Google Scholar]
  25. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J.J. ( 2009;). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59 992997 [View Article] [PubMed].
    [Google Scholar]
  26. Suriyachadkun C., Ngaemthao W., Chunhametha S., Tamura T., Sanglier J.J. ( 2013;). Kutzneria buriramensis sp. nov., isolated from soil, and emended description of the genus Kutzneria . Int J Syst Evol Microbiol 63 4752 [View Article] [PubMed].
    [Google Scholar]
  27. Suzuki S., Okuda T., Komatsubara S. ( 2001;). Selective isolation and study on the global distribution of the genus Planobispora in soils. Can J Microbiol 47 979986 [View Article] [PubMed].
    [Google Scholar]
  28. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [View Article].
    [Google Scholar]
  29. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. ( 1994;). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44 193203 [View Article] [PubMed].
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  31. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 46734680 [View Article] [PubMed].
    [Google Scholar]
  32. Uchida K., Aida K. ( 1984;). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30 131134 [View Article].
    [Google Scholar]
  33. Verlander C.P. ( 1992;). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185201. Edited by Kricka L. J. New York: Academic Press;. [CrossRef]
    [Google Scholar]
  34. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  35. Williams S.T., Cross T. ( 1971;). Actinomycetes. Methods Microbiol 4 295334. [CrossRef]
    [Google Scholar]
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