Description of sp. nov., a producer of antibiotics against microbial pathogens, isolated from soybean () rhizosphere soil Free

Abstract

An actinomycete, designated strain T66 and isolated from soybean rhizosphere soil at Gyeonggi Siheung Sorae in the Republic of Korea, has antibiotic activity against a broad range of microbial pathogens. The strain was determined to be closely related to several known species in the genus on the basis of 16S rRNA gene sequence data (97.73–98.07 % similarity). The strain exhibited cell-wall chemotype I and phospholipid type II. The menaquinones present were MK-9 (H), MK-9 (H) and MK-10 (H). Major fatty acids were anteiso-C, iso-C, iso-C, and anteiso-C. The level of DNA–DNA relatedness between strain T66 and closely related type strains was determined to be below 40 %. Strain T66 had spiral spore chains and a rugose spore surface that is different from its closest relatives. Comparison of the genotypic and phenotypic features confirmed that strain T66 ( = KEMB 9005-219 = KACC 18226 = NBRC 110902) should be considered as the type strain of a novel species in the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • Ministry of Agriculture, Food and Rural Affairs (Award (312027-3)
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2015-11-01
2024-03-29
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