1887

Abstract

The taxonomic position of an actinobacterium strain, C296001, isolated from a soil sample collected in Sarawak, Malaysia, was established using a polyphasic approach. Phylogenetically, strain C296001 was closely associated with the genus and formed a distinct monophyletic clade with the only described species, NBRC 105296. The 16S rRNA gene sequence similarity between strain C296001 and was 98.7 %. DNA–DNA hybridization results showed that the relatedness of strain C296001 to was only 21.5 %. The DNA G+C content of strain C296001 was 71.7 mol%. Using a PacBio RS II system, whole genome sequences for strains C296001 and NBRC 105296 were obtained. The genome sizes of 4.5 Mbp and 5.4 Mbp determined were similar to those of other members of the family . The cell-wall peptidoglycan contained lysine, alanine, aspartic acid, glutamic acid and serine, representing the peptidoglycan type A4α -Lys--Ser--Asp. The major menaquinones were MK-8(H), MK-8 and MK-8(H). Phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and phosphoglycolipid were the polar lipids, while the whole-cell sugars were glucose, fucose and lesser amounts of ribose and galactose. The major fatty acids were iso-C, anteiso-C, iso-C H, anteiso-Cω9, iso-C and 10-methyl C. Chemotaxonomic analyses showed that C296001 had typical characteristics of members of the genus with the main differences occurring in phenotypic characteristics. On the basis of the phenotypic and chemotaxonomic evidence, it is proposed that strain C296001 be classified as a representative of a novel species in the genus , for which the name sp. nov. is recommended. The type strain is C296001 ( = ATCC TSD-4 = JCM 30660).

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2015-11-01
2019-10-19
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