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Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped strain, THG-DN7.3, was isolated from a waterfall. Strain THG-DN7.3 grew well at 18–28 °C and at pH 6.0–7.5 on Reasoner's 2A agar. Based on 16S rRNA gene sequence comparisons, strain THG-DN7.3 was most closely related to JS4-4 (97.3 % 16S rRNA gene sequence similarity) and SHS5-24 (96.5 %). The G+C content of the genomic DNA was 57.4 mol%. The mean DNA–DNA relatedness of strain THG-DN7.3 with KACC 12607 was 40 ± 1 % (reciprocal 50 ± 2.1 %). The major cellular fatty acids of strain THG-DN7.3 were summed feature 3 (Cω7 and/or Cω6) (47.4 %), C (30.4 %), summed feature 8 (Cω6 and/or Cω7) (6.8 %) and C (6.2 %). The predominant isoprenoid quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of the DNA–DNA hybridization and genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-DN7.3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-DN7.3 ( = KCTC 42243 = CCTCC AB 2015119).

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2015-11-01
2019-10-16
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