1887

Abstract

A Gram-stain-negative, non-motile, aerobic bacterium, strain 13-93-B1, was isolated from seawater off Jeju Island, Republic of Korea, and was subjected to polyphasic taxonomic study. Cells formed ivory colonies and were ovoid to rod-shaped. The strain was catalase-positive, oxidase-negative and grew optimally at 30 °C, in the presence of 1–2 % (w/v) NaCl and at pH 7.0–7.5. It did not synthesize bacteriochlorophyll . Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain 13-93-B1 clustered with the type strain SW-277 (97.0 % 16S rRNA gene sequence similarity). DNA–DNA hybridization between strain 13-93-B1 and KCTC 12735 was 33.1 ± 1.4 % (35.2 ± 2.8 % in a reciprocal experiment). The predominant cellular fatty acid was summed feature 8 (Cω7/Cω6; 76.9 %). The major respiratory quinone was ubiquinone Q-10 and polar lipids detected in strain 13-93-B1 were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and unidentified lipids. The DNA G+C content of strain 13-93-B1 was 60.4 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-93-B1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 13-93-B1 ( = DSM 100212 = KCTC 42571)

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2015-11-01
2024-03-29
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