1887

Abstract

A Gram-stain-negative, non-motile, aerobic bacterium, strain 13-93-B1, was isolated from seawater off Jeju Island, Republic of Korea, and was subjected to polyphasic taxonomic study. Cells formed ivory colonies and were ovoid to rod-shaped. The strain was catalase-positive, oxidase-negative and grew optimally at 30 °C, in the presence of 1–2 % (w/v) NaCl and at pH 7.0–7.5. It did not synthesize bacteriochlorophyll . Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain 13-93-B1 clustered with the type strain SW-277 (97.0 % 16S rRNA gene sequence similarity). DNA–DNA hybridization between strain 13-93-B1 and KCTC 12735 was 33.1 ± 1.4 % (35.2 ± 2.8 % in a reciprocal experiment). The predominant cellular fatty acid was summed feature 8 (Cω7/Cω6; 76.9 %). The major respiratory quinone was ubiquinone Q-10 and polar lipids detected in strain 13-93-B1 were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and unidentified lipids. The DNA G+C content of strain 13-93-B1 was 60.4 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-93-B1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 13-93-B1 ( = DSM 100212 = KCTC 42571)

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2015-11-01
2019-12-08
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References

  1. Baumann P., Baumann L.. ( 1981;). The marine Gram staining negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G.. Berlin: Springer;.
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  3. Buchan A., González J.M., Moran M.A.. ( 2005;). Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71: 5665–5677 [CrossRef] [PubMed].
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W.R., Stanier R.Y.. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49: 25–68 [CrossRef] [PubMed].
    [Google Scholar]
  5. Cowan S.T., Steel K.J.. ( 1965;). Manual for the Identification of Medical Bacteria London: Cambridge University Press;.
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224–229 [CrossRef].
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  9. Fitch W.M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  10. Garrity G.M., Bell J.A., Lilburn T.. ( 2005;). Family I. Rhodobacteraceae fam. nov. . In Bergey's Manual of Systematic Bacteriologyvol 2c, 2nd edn.., p. 161. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York: Springer;.
    [Google Scholar]
  11. Giovannoni S., Rappé M.. ( 2000;). Evolution, diversity and molecular ecology of marine prokaryotes. . In Microbial Ecology of the Oceans, pp. 47–84. Edited by Kirchman D. L.. New York: Wiley;.
    [Google Scholar]
  12. Hameed A., Shahina M., Lin S.-Y., Nakayan P., Liu Y.-C., Lai W.-A., Hsu Y.-H.. ( 2014;). Youngimonas vesicularis gen. nov., sp. nov., of the family Rhodobacteraceae, isolated from surface seawater, reclassification of Donghicola xiamenensis Tan et al. 2009 as Pseudodonghicola xiamenensis gen. nov., comb. nov. and emended description of the genus Donghicola Yoon et al. 2007. Int J Syst Evol Microbiol 64: 2729–2737 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–207 [CrossRef].
    [Google Scholar]
  15. Lyman J., Fleming R.H.. ( 1940;). Composition of sea water. J Mar Res 3: 134–146.
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W.B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  17. Minnikin D.E., Patel P.V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27: 104–117 [CrossRef].
    [Google Scholar]
  18. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  19. Rappé M.S., Vergin K., Giovannoni S.J.. ( 2000;). Phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems. FEMS Microbiol Ecol 33: 219–232 [CrossRef] [PubMed].
    [Google Scholar]
  20. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  21. Sambrook J., Russell D.W.. ( 2001;). Molecular Cloning: a Laboratory Manual, 3rd edn.. Cold Spring Harbor: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  22. Sasser M.. ( 2001;). Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark: MIDI Inc;.
    [Google Scholar]
  23. Staley J.T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95: 1921–1942 [PubMed].
    [Google Scholar]
  24. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25: 125–128 [CrossRef].
    [Google Scholar]
  25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  26. Tan T., Wang B., Shao Z.. ( 2009;). Donghicola xiamenensis sp. nov., a marine bacterium isolated from seawater of the Taiwan Strait in China. Int J Syst Evol Microbiol 59: 1143–1147 [CrossRef] [PubMed].
    [Google Scholar]
  27. Thompson J.D., Higgins D.G., Gibson T.J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680 [CrossRef] [PubMed].
    [Google Scholar]
  28. Wang B., Tan T., Shao Z.. ( 2009;). Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 59: 1116–1121 [CrossRef] [PubMed].
    [Google Scholar]
  29. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  30. Wood P.J., Erfle J.D., Teather R.M.. ( 1988;). Use of complex formation between Congo red and polysaccharides in detection and assay of polysaccharide hydrolases. Methods Enzymol 160: 59–74 [CrossRef].
    [Google Scholar]
  31. Yang S.-H., Kwon K.K., Lee H.-S., Kim S.-J.. ( 2006;). Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56: 2879–2882 [CrossRef] [PubMed].
    [Google Scholar]
  32. Yoon J.-H., Kang S.-J., Oh T.-K.. ( 2007;). Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57: 73–76 [CrossRef] [PubMed].
    [Google Scholar]
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