1887

Abstract

A Gram-stain-positive, aerobic, non-motile, curved (selenoid), rod-shaped actinobacterium, designated KNC, was isolated from the 0.2 μm-filtrate of river water in western Japan. Cells of strain KNC were ultramicrosized (0.04–0.05 μm). The strain grew at 15–37 °C, with no observable growth at 10 °C or 40 °C. The pH range for growth was 7–9, with weaker growth at pH 10. Growth was impeded by the presence of NaCl at concentrations greater than 1 %. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KNC showed relatively high sequence similarity (97.2 %) to Cr8-25 in the family . However, strain KNC formed an independent cluster with cultured, but as-yet-unidentified, species and environmental clones on the phylogenetic tree. The major cellular fatty acids were anteiso-C (41.0 %), iso-C (21.8 %), C (18.0 %) and anteiso-C (12.9 %), and the major menaquinones were MK-11 (71.3 %) and MK-12 (13.6 %). The major polar lipids were phosphatidylglycerol and two unknown glycolipids. The cell-wall muramic acid acyl type was acetyl. The peptidoglycan was B-type, and contained 3-hydroxyglutamic acid, glutamic acid, aspartic acid, glycine, alanine and lysine, with the latter being the diagnostic diamino acid. The G+C content of the genome was unusually low for actinobacteria (52.1 mol%), compared with other genera in the family . Based on the phenotypic characteristics and phylogenetic evidence, strain KNC represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is KNC ( = NBRC 105389 = NCIMB 14875).

Funding
This study was supported by the:
  • , Japan Society for the Promotion of Science , (Award 23570117)
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2015-11-01
2021-02-26
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