1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_11

sp. nov., isolated from rhizosphere soil of a potato field Free

Abstract

A novel Gram-stain-positive, endospore-forming bacterium, designated strain FJAT-18043, was isolated from a soil sample of a potato field in Xinjiang Autonomous Region, China. Cells were rods that were catalase-positive and motile by peritrichous flagella. The strain grew at 20–45 °C (optimum 35 °C), at pH 6.0–10.0 (optimum pH 9) and with 0–10 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-18043 belonged to the genus and exhibited similarities of 97.7, 97.6, 97.2 and 97.2 % with A1-2, DSM 23495, WCC 4585 and DS22, respectively. DNA–DNA relatedness between strain FJAT-18043 and A1-2 was lower than 70 % (36.1 %). The menaquinone was identified as MK-7 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids detected were anteiso-C, iso-C, iso-C, anteiso-C, C and iso-C. The DNA G+C content was 48.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-18043 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is FJAT-18043 ( = DSM 29501 = CCTCC AB 2014277).

  • Published Online:
Funding
This study was supported by the:
  • the international cooperation project of the Chinese Ministry of Science and Technology (Award 2012DFA31120)
  • Natural Science Foundation of China (NSFC) (Award 31370059)
  • doctor fund of Fujian Academy of Agricultural Sciences (Award 2014BS-3)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000539
2015-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/4066.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000539&mimeType=html&fmt=ahah

References

  1. Albert R. A., Archambault J., Rosselló-Mora R., Tindall B. J., Matheny M. ( 2005;). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55 2125–2130 [View Article] [PubMed].
     [Google Scholar]
  2. Atlas R. M. ( 1993;). Handbook of Microbiological Media. . Edited by Parks L. C. Boca Raton, FL: CRC Press;.
     [Google Scholar]
  3. Buck J. D. ( 1982;). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44 992–993 [PubMed].
     [Google Scholar]
  4. Chen Y. G., Cui X. L., Pukall R., Li H. M., Yang Y. L., Xu L. H., Wen M. L., Peng Q., Jiang C. L. ( 2007;). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57 2327–2332 [View Article] [PubMed].
     [Google Scholar]
  5. Claus D., Berkeley R. C. W. ( 1986;). Genus Bacillus. . In Bergey's Manual of Systematic Bacteriology, pp. 1105–1139. Edited by Sneath P. H., Mair N., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;.
     [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221–230 [View Article] [PubMed].
     [Google Scholar]
  7. Cowan S. T., Steel K. J. ( 1965). Manual for the Identification of Medical Bacteria., London: Cambridge University Press;.
     [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133–142 [View Article] [PubMed].
     [Google Scholar]
  9. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  10. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–789 [View Article].
     [Google Scholar]
  11. Fitch W. M. ( 1971;). Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  12. Gao J. L., Sun J. G., Li Y., Wang E. T., Chen W. X. ( 1994;). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol 44 151–158 [View Article].
     [Google Scholar]
  13. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996;). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46 234–239 [View Article] [PubMed].
     [Google Scholar]
  14. Hong S. W., Park J. M., Kim S.-J., Chung K. S. ( 2012;). Bacillus eiseniae sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm (Eisenia fetida L.). Int J Syst Evol Microbiol 62 2077–2083 [View Article] [PubMed].
     [Google Scholar]
  15. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. (editors) ( 1985). Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;.
     [Google Scholar]
  16. Huss V. A., Festl H., Schleifer K. H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184–192 [View Article] [PubMed].
     [Google Scholar]
  17. Jukes T. H., Cantor C. R. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N. vol. 3 New York: Academic Press; [View Article].
     [Google Scholar]
  18. Kämpfer P. ( 1994;). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17 86–98 [View Article].
     [Google Scholar]
  19. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  20. Lee J. S., Shin Y. K., Yoon J. H., Takeuchi M., Pyun Y. R., Park Y. H. ( 2001;). Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 51 1491–1498 [PubMed]. [CrossRef]
    [Google Scholar]
  21. Lei Z., Qiu P., Ye R., Tian J., Liu Y., Wang L., Tang S. K., Li W. J., Tian Y. ( 2014;). Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil. J Gen Appl Microbiol 60 101–105 [View Article] [PubMed].
     [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  24. Murray R. G. E., Doetsch R. N., Robinow C. F. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  25. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  26. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News Lett 20 1–6.
     [Google Scholar]
  27. Schumann P. ( 2011;). Peptidoglycan structure. Methods Microbiol 38 101–129 [View Article].
     [Google Scholar]
  28. Seiler H., Wenning M., Schmidt V., Scherer S. ( 2013;). Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site. Int J Syst Evol Microbiol 63 867–872 [View Article] [PubMed].
     [Google Scholar]
  29. Smibert R. M., Krieg N. R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega 6: molecular evolutionary genetics analysis 6.0. Mol Biol Evol 30 2725–2729 [View Article] [PubMed].
     [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  32. Vaishampayan P., Probst A. J., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K. ( 2010;). Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. Int J Syst Evol Microbiol 60 1031–1037. [CrossRef]
    [Google Scholar]
  33. Vaz-Moreira I., Figueira V., Lopes A. R., Lobo-da-Cunha A., Spröer C., Schumann P., Nunes O. C., Manaia C. M. ( 2012;). Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant. Int J Syst Evol Microbiol 62 71–77 [View Article] [PubMed].
     [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
  35. Zhang L., Wang Y., Dai J., Tang Y., Yang Q., Luo X., Fang C. ( 2009;). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. Int J Syst Evol Microbiol 59 1787–1792 [View Article] [PubMed].
     [Google Scholar]
  36. Zhang L., Wu G. L., Wang Y., Dai J., Fang C. X. ( 2011;). Bacillus deserti sp. nov., a novel bacterium isolated from the desert of Xinjiang, China. Antonie van Leeuwenhoek 99 221–229 [View Article] [PubMed].
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000539
Loading
Bacillus solani sp. nov., isolated from rhizosphere soil of a potato field
Int J Syst Evol Microbiol 65, 4066 (2015); https://doi.org/10.1099/ijsem.0.000539
/content/journal/ijsem/10.1099/ijsem.0.000539
/content/journal/ijsem/10.1099/ijsem.0.000539
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error