1887

Abstract

A Gram-stain-negative, yellow-pigmented bacterial strain, designated PR1012K, was isolated from a motor car evaporator core collected in Korea. Cells of the strain were facultatively anaerobic, non-spore-forming and rod-shaped. The strain grew at 10–40 °C (optimum, 25 °C), at pH 6.5–8.0 (optimum, pH 7.0–8.0) and in the presence of 0–1 % (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus (97.50–90.74 % 16S rRNA gene sequence similarities) and showed highest sequence similarity to DSM 21099 (97.50 %). Its predominant fatty acids included summed feature 3 (Cω7 and/or Cω6), Cω5, iso-C and summed feature 4 (iso-C I and/or anteiso B) and it had MK-7 as the major menaquinone. The polar lipids present included phosphatidylethanolamine, one unknown aminophospholipid, two unknown aminolipids and five unknown polar lipids. The DNA G+C content of this strain was 54 mol%. Based on phenotypic, genotypic and chemotaxonomic data, strain PR1012K represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is PR1012K ( = KACC 17939 = NBRC 110794).

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2015-11-01
2024-04-19
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References

  1. Ahn J. H., Weon H. Y., Kim S. J., Hong S. B., Seok S. J., Kwon S. W. ( 2014;). Spirosoma oryzae sp. nov., isolated from rice soil and emended description of the genus Spirosoma. Int J Syst Evol Microbiol 64 32303234 [View Article] [PubMed].
    [Google Scholar]
  2. Baik K. S., Kim M. S., Park S. C., Lee D. W., Lee S. D., Ka J. O., Choi S. K., Seong C. N. ( 2007;). Spirosoma rigui sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 57 28702873 [View Article] [PubMed].
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B. ( 2002;). Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  4. Chang X., Jiang F., Wang T., Kan W., Qu Z., Ren L., Fang C., Peng F. ( 2014;). Spirosoma arcticum sp. nov., isolated from high Arctic glacial till. Int J Syst Evol Microbiol 64 22332237 [View Article] [PubMed].
    [Google Scholar]
  5. Counsell T. J., Murray R. G. E. ( 1986;). Polar lipid profiles of the genus Deinococcus . Int J Syst Bacteriol 36 202206 [View Article].
    [Google Scholar]
  6. Denner E. B., Paukner S., Kämpfer P., Moore E. R., Abraham W. R., Busse H. J., Wanner G., Lubitz W. ( 2001;). Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan. Int J Syst Evol Microbiol 51 827841 [View Article] [PubMed].
    [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  8. Finster K. W., Herbert R. A., Lomstein B. A. ( 2009;). Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma. Int J Syst Evol Microbiol 59 839844 [View Article] [PubMed].
    [Google Scholar]
  9. Fitch W. M. ( 1971;). Toward defining course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  10. Fries J., Pfeiffer S., Kuffner M., Sessitsch A. ( 2013;). Spirosoma endophyticum sp. nov., isolated from Zn- and Cd-accumulating Salix caprea. Int J Syst Evol Microbiol 63 45864590 [View Article] [PubMed].
    [Google Scholar]
  11. Gonzalez J. M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773 [View Article] [PubMed].
    [Google Scholar]
  12. Jukes T. H., Cantor C. R. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, [View Article] pp. 21132. Edited by Munro H. N. 3 New York: Academic Press;.
    [Google Scholar]
  13. Kim D. U., Ka J. O. ( 2014;). Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 64 10241029 [View Article] [PubMed].
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  15. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [View Article].
    [Google Scholar]
  16. Larkin J. M., Borrall R. ( 1984;). Spirosomaceae Larkin and Borrall 1978, 595AL . . In Bergey's Manual of Systematic Bacteriology, pp. 125126. Edited by Krieg N. R., Holt J. G. 1 Baltimore: Williams & Wilkins;.
    [Google Scholar]
  17. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  19. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  20. Ten L. N., Xu J. L., Jin F. X., Im W. T., Oh H. M., Lee S. T. ( 2009;). Spirosoma panaciterrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 59 331335 [View Article] [PubMed].
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 46734680 [View Article] [PubMed].
    [Google Scholar]
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