1887

Abstract

Seven Gram-staining-negative, strictly aerobic, pale-yellow-pigmented, rod-shaped and non-motile strains were isolated from the sea urchin collected from Troitsa Bay, Sea of Japan. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were affiliated with the family . The novel isolates showed 99.9–100 % 16S rRNA gene sequence similarity to each other and were closely related to the type strains of the recognized members of the genus with sequence similarities of 95.8–98.4 %. The G+C content of the genomic DNA was 35–36 mol%. DNA–DNA relatedness among the sea urchin isolates was 95–99 % and between strain KMM 6277 and its most closely related type strains, KCTC 23842 and JCM 13034, was 38 and 27 %, respectively. The prevalent fatty acids were iso-C, anteiso-C, summed feature 3 (comprising iso-C 2-OH and/or C ω7 fatty acids), iso-C and C. The polar lipid profile was composed of the phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. The main respiratory isoprenoid quinone was MK-6.The results of phylogenetic, phenotypic and genotypic analyses indicated that the novel strains represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is KMM 6277 ( = CCUG 62221 = LMG 26737).

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2015-11-01
2019-10-14
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