1887

Abstract

Seven Gram-staining-negative, strictly aerobic, pale-yellow-pigmented, rod-shaped and non-motile strains were isolated from the sea urchin collected from Troitsa Bay, Sea of Japan. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were affiliated with the family . The novel isolates showed 99.9–100 % 16S rRNA gene sequence similarity to each other and were closely related to the type strains of the recognized members of the genus with sequence similarities of 95.8–98.4 %. The G+C content of the genomic DNA was 35–36 mol%. DNA–DNA relatedness among the sea urchin isolates was 95–99 % and between strain KMM 6277 and its most closely related type strains, KCTC 23842 and JCM 13034, was 38 and 27 %, respectively. The prevalent fatty acids were iso-C, anteiso-C, summed feature 3 (comprising iso-C 2-OH and/or C ω7 fatty acids), iso-C and C. The polar lipid profile was composed of the phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. The main respiratory isoprenoid quinone was MK-6.The results of phylogenetic, phenotypic and genotypic analyses indicated that the novel strains represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is KMM 6277 ( = CCUG 62221 = LMG 26737).

Funding
This study was supported by the:
  • Russian Science Foundation (Award 14-50-00034)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000525
2015-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3991.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000525&mimeType=html&fmt=ahah

References

  1. Balakirev E. S., Pavlyuchkov V. A., Ayala F. J. ( 2008;). DNA variation and symbiotic associations in phenotypically diverse sea urchin Strongylocentrotus intermedius . Proc Natl Acad Sci U S A 105 1621816223 [View Article] [PubMed].
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  3. Bligh E. G., Dyer W. J. ( 1959;). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37 911917 [View Article] [PubMed].
    [Google Scholar]
  4. Choi D. H., Cho B. C. ( 2006;). Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. Int J Syst Evol Microbiol 56 771776 [View Article] [PubMed].
    [Google Scholar]
  5. Choi A., Yang S.-J., Cho J.-C. ( 2013;). Lutibacter flavus sp. nov., a marine bacterium isolated from a tidal flat sediment. Int J Syst Evol Microbiol 63 946951 [View Article] [PubMed].
    [Google Scholar]
  6. Collins M. D., Shah H. M. ( 1984;). Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa . Arch Microbiol 137 247249 [View Article].
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. ( 1969;). Evolution of protein molecules. . In Mamalian Protein Metabolism, pp. 21132. Edited by Munro H. N. 3 New York: Academic Press; [View Article].
    [Google Scholar]
  9. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161207 [View Article].
    [Google Scholar]
  10. Lee S.-Y., Lee M. H., Oh T.-K., Yoon J.-H. ( 2012;). Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006.. Int J Syst Evol Microbiol 62 420424 [View Article] [PubMed].
    [Google Scholar]
  11. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  12. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [View Article] [PubMed].
    [Google Scholar]
  13. Nedashkovskaya O. I., Van Trappen S., Frolova G. M., De Vos P. ( 2012;). Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . Arch Microbiol 194 215221 [View Article] [PubMed].
    [Google Scholar]
  14. Park S., Kang S. J., Oh T. K., Yoon J. H. ( 2010;). Lutibacter maritimus sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 60 610614 [View Article] [PubMed].
    [Google Scholar]
  15. Park S. C., Choe H. N., Hwang Y. M., Baik K. S., Seong C. N. ( 2013;). Lutibacter agarilyticus sp. nov., a marine bacterium isolated from shallow coastal seawater. Int J Syst Evol Microbiol 63 26782683 [View Article] [PubMed].
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  17. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20 16.
    [Google Scholar]
  18. Sung H.-R., Shin K.-S., Ghim S.-Y. ( 2015;). Lutibacter oricola sp. nov., a marine bacterium isolated from seawater. Int J Syst Evol Microbiol 65 485490 [View Article] [PubMed].
    [Google Scholar]
  19. Vancanneyt M., Naser S. M., Engelbeen K., De Wachter M., Van der Meulen R., Cleenwerck I., Hoste B., De Vuyst L., Swings J. ( 2006;). Reclassification of Lactobacillus brevis strains LMG 11494 and LMG 11984 as Lactobacillus parabrevis sp. nov. Int J Syst Evol Microbiol 56 15531557 [View Article] [PubMed].
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler P., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000525
Loading
/content/journal/ijsem/10.1099/ijsem.0.000525
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error