1887

Abstract

A brown, moderately halophilic, photoautotrophic bacterium designated strain JA363 was purified from a photoheterotrophic enrichment obtained from sediment from Chilika lagoon, Odisha, India. Cells of the isolate were coccoid, motile by means of single polar flagellum and Gram-stain-negative. Strain JA363 had an obligate requirement for NaCl and could tolerate up to 7 % (w/v) NaCl. Strain JA363 had complex growth factor requirements. Internal photosynthetic membranes were present as vesicles. Strain JA363 contained bacteriochlorophyll and spirilloxanthin series carotenoids with rhodopin as a major (>85 %) component. Cω7/Cω6, Cω7 and C were the major fatty acids and phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. Q8 was the predominant quinone system of strain JA363. The DNA G+C content was 64 mol%. The highest 16S rRNA gene sequence similarity of strain JA363 was found with the type strains of (98.7 %), (98.2 %), (97.4 %) and (96.3 %). However, the phylogenetic trees generated firmly placed strain JA363 in the genus , which was further supported by phenotypic and chemotaxonomic evidence. Consequently, strain JA363 is described as representing a novel species of the genus as sp. nov. The type strain is JA363 ( = KCTC 5701 = NBRC 104959).

Funding
This study was supported by the:
  • , University Grants Commission
  • , Department of Science and Technology
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000517
2015-11-01
2021-03-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3938.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000517&mimeType=html&fmt=ahah

References

  1. Anil Kumar P., Sasi Jyothsna T. S., Srinivas T. N. R., Sasikala Ch, Ramana Ch. V., Imhoff J. F. ( 2007;). Two novel species of marine phototrophic Gammaproteobacteria and Thiorhodococcus bheemlicus sp. nov. and Thiorhodococcus kakinadensis sp. nov. Int J Syst Evol Microbiol 57 24582461 [CrossRef] [PubMed].
    [Google Scholar]
  2. Anil Kumar P., Srinivas T. N. R., Sasikala Ch., Ramana Ch. V., Imhoff J. F. ( 2008;). Thiophaeococcus mangrovi gen. nov., sp. nov., a photosynthetic, marine gammaproteobacterium isolated from the Bhitarkanika mangrove forest of India. Int J Syst Evol Microbiol 58 26602664 [CrossRef] [PubMed].
    [Google Scholar]
  3. Divyasree B., Lakshmi K. V. N. S., Sasikala Ch, Ramana Ch. V. ( 2014;). Thiophaeococcus fuscus sp. nov., isolated from a lagoon. Int J Syst Evol Microbiol 64 25282533 [CrossRef] [PubMed].
    [Google Scholar]
  4. Guyoneaud R., Matheron R., Liesack W., Imhoff J. F., Caumette P. ( 1997;). Thiorhodococcus minus, gen. nov., sp. nov., a new purple sulfur bacterium isolated from coastal lagoon sediments. Arch Microbiol 168 1623 [CrossRef] [PubMed].
    [Google Scholar]
  5. Hanada S., Takaichi S., Matsuura K., Nakamura K. ( 2002;). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52 187193 [PubMed]. [CrossRef]
    [Google Scholar]
  6. Hiraishi A., Hoshino Y. ( 1984;). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30 435448 [CrossRef].
    [Google Scholar]
  7. Hiraishi A., Hoshino Y., Kitamura H. ( 1984;). Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 30 197210 [CrossRef].
    [Google Scholar]
  8. Imhoff J. F. ( 1984;). Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25 8589 [CrossRef].
    [Google Scholar]
  9. Imhoff J. F., Bias-Imhoff U. ( 1995;). Lipids, quinones and fatty acids of anoxygenic phototrophic bacteria. . In Anoxygenic Photosynthetic Bacteria, pp. 179205. Edited by Blackenship R. E., Madigan M. T., Bauer C. E. vol. 2 Dordrecht: Kluwer Academic Publishers;. [CrossRef]
    [Google Scholar]
  10. Imhoff J. F., Caumette P. ( 2004;). Recommended standards for the description of new species of anoxygenic phototrophic bacteria. Int J Syst Evol Microbiol 54 14151421 [CrossRef] [PubMed].
    [Google Scholar]
  11. Imhoff J. F., Kushner D. J., Kushwaha S. C., Kates M. ( 1982;). Polar lipids in phototrophic bacteria of the Rhodospirillaceae and Chromatiaceae families. J Bacteriol 150 11921201 [PubMed].
    [Google Scholar]
  12. Imhoff J. F., Süling J., Petri R. ( 1998;). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . Int J Syst Bacteriol 48 11291143 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kates M. ( 1972;). Techniques in lipidology. . In Laboratory Techniques in Biochemistry and Molecular Biology, pp. 355356. Edited by Work T. S., Work E. vol. 3 New York: American Elsevier;.
    [Google Scholar]
  14. Kates M. ( 1986;). Techniques of lipidology: isolation, analysis and identification of lipids. . In Laboratory Techniques in Biochemistry and Molecular Biology, pp. 100112. Edited by Burdon R. H., van Knippenberg P. H. vol. 3 Amsterdam: Elsevier;.
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [CrossRef] [PubMed].
    [Google Scholar]
  17. Lakshmi K. V. N. S., Sasikala Ch., Takaichi S., Ramana Ch. V. ( 2011;). Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 61 16561661 [CrossRef] [PubMed].
    [Google Scholar]
  18. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [CrossRef].
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [CrossRef].
    [Google Scholar]
  20. Oren A., Duker S., Ritter S. ( 1996;). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138 135140 [CrossRef].
    [Google Scholar]
  21. Rabold S., Gorlenko V. M., Imhoff J. F. ( 2006;). Thiorhodococcus mannitoliphagus sp. nov., a purple sulfur bacterium from the White Sea. Int J Syst Evol Microbiol 56 19451951 [CrossRef] [PubMed].
    [Google Scholar]
  22. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  23. Shivali K., Ramana V. V., Ramaprasad E. V., Sasikala Ch., Ramana Ch. V. ( 2011;). Marichromatium litoris sp. nov. and Marichromatium chrysaorae sp. nov. isolated from beach sand and from a jelly fish (Chrysaora colorata). Syst Appl Microbiol 34 600605 [CrossRef] [PubMed].
    [Google Scholar]
  24. Stackebrandt E., Goebel B. M. ( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849. [CrossRef]
    [Google Scholar]
  25. Subhash Y., Sasikala Ch., Ramana Ch. V. ( 2013a;). Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 63 34633469 [CrossRef] [PubMed].
    [Google Scholar]
  26. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013b;). Mongoliicoccus alkaliphilus sp. nov. and Litoribacter alkaliphilus sp. nov., isolated from salt pans. Int J Syst Evol Microbiol 63 34573462 [CrossRef] [PubMed].
    [Google Scholar]
  27. Sucharita K., Sasikala C., Ramana Ch. V. ( 2010;). Thiorhodococcus modestalkaliphilus sp. nov. a phototrophic gammaproteobacterium from Chilika salt water lagoon, India. J Gen Appl Microbiol 56 9399 [CrossRef] [PubMed].
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed].
    [Google Scholar]
  29. Tindall B. J. ( 1990a;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199202 [CrossRef].
    [Google Scholar]
  30. Tindall B. J. ( 1990b;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13 128130 [CrossRef].
    [Google Scholar]
  31. Tourova T. P., Antonov A. S. ( 1987;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19 333355. [CrossRef]
    [Google Scholar]
  32. Trüper H. G., Pfennig N. ( 1981;). Characterization and identification of the anoxygenic phototrophic bacteria. [CrossRef]. In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 299312. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. vol. 1 New York: Springer;.
    [Google Scholar]
  33. Zaar A., Fuchs G., Golecki J. R., Overmann J. ( 2003;). A new purple sulfur bacterium isolated from a littoral microbial mat, Thiorhodococcus drewsii sp. nov. Arch Microbiol 179 174183 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000517
Loading
/content/journal/ijsem/10.1099/ijsem.0.000517
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error