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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_11

sp. nov., a gene-harbouring endophytic bacterium isolated from maize root Free

Abstract

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain 522, was isolated from surface-sterilized root tissue of maize planted in Fangshan District of Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belonged to the genus and showed less than 93.9 % similarity to the type strains of all recognized species of the genus . The predominant respiratory quinone was menaquinone-6 and the polar lipid profile was composed of the major lipids phosphatidylethanolamine, phosphatidylserine and two unidentified amino lipids. The major fatty acids were C, iso-C, iso-C G and iso-C.The G+C content of the DNA was 37.7 mol%. The results of physiological and biochemical tests and the differences in fatty acid profiles allowed the clear phenotypic differentiation of strain 522 from closely related species of the genus Strain 522 therefore represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 522 ( = ACCC 19708 = DSM 29537).

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Funding
This study was supported by the:
  • Special Fund for Agro-scientific Research in the Public Interest (Award 201203045)
  • National Natural Science Foundation of China (Award 31570008)
© 2015 IUMS
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2015-11-01
2024-04-20
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Flavobacterium endophyticum sp. nov., a nifH gene-harbouring endophytic bacterium isolated from maize root
Int J Syst Evol Microbiol 65, 3900 (2015); https://doi.org/10.1099/ijsem.0.000513
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