1887

Abstract

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain 522, was isolated from surface-sterilized root tissue of maize planted in Fangshan District of Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belonged to the genus and showed less than 93.9 % similarity to the type strains of all recognized species of the genus . The predominant respiratory quinone was menaquinone-6 and the polar lipid profile was composed of the major lipids phosphatidylethanolamine, phosphatidylserine and two unidentified amino lipids. The major fatty acids were C, iso-C, iso-C G and iso-C.The G+C content of the DNA was 37.7 mol%. The results of physiological and biochemical tests and the differences in fatty acid profiles allowed the clear phenotypic differentiation of strain 522 from closely related species of the genus Strain 522 therefore represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 522 ( = ACCC 19708 = DSM 29537).

Funding
This study was supported by the:
  • Special Fund for Agro-scientific Research in the Public Interest (Award 201203045)
  • National Natural Science Foundation of China (Award 31570008)
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2015-11-01
2021-10-20
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References

  1. Ali Z., Cousin S., Frühling A., Brambilla E., Schumann P., Yang Y., Stackebrandt E. ( 2009;). Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet. Int J Syst Evol Microbiol 59 26102617 [View Article] [PubMed].
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. ), ( 1923;). Genus II. Flavobacterium gen. nov.. Bergey's Manual of Determinative Bacteriology, pp. 97117 Baltimore: Williams & Wilkins;.
    [Google Scholar]
  3. Bernardet J. F., Bowman J. P. ( 2011;). Genus I. Flavobacterium Bergey et al. 1923. . In Bergey's Manual of Systematic Bacteriology, pp. 112154. Edited by Whitman W. vol. 4 , 2nd edn.., Baltimore: Williams & Wilkins;.
    [Google Scholar]
  4. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. ( 1996;). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46 128148 [View Article].
    [Google Scholar]
  5. Bernardet J.-F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  6. Collins M., Jones D. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Microbiol 48 459470.
    [Google Scholar]
  7. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed].
    [Google Scholar]
  8. Cousin S., Päuker O., Stackebrandt E. ( 2007;). Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. Int J Syst Evol Microbiol 57 243249 [View Article] [PubMed].
    [Google Scholar]
  9. Ding Y., Wang J., Liu Y., Chen S. ( 2005;). Isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in Beijing region. J Appl Microbiol 99 12711281 [View Article] [PubMed].
    [Google Scholar]
  10. Dong K., Chen F., Du Y., Wang G. ( 2013;). Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense . Int J Syst Evol Microbiol 63 886892 [View Article] [PubMed].
    [Google Scholar]
  11. Fautz E., Reichenbach J. R. ( 1980;). A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8 8791 [View Article].
    [Google Scholar]
  12. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  13. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  14. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. ), ( 1994). Methods for General and Molecular Bacteriology., Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  15. Joung Y., Kim H., Ahn T. S., Joh K. ( 2012;). Flavobacterium yonginense sp. nov. and Flavobacterium myungsuense sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 62 806810 [View Article] [PubMed].
    [Google Scholar]
  16. Kang J. Y., Chun J., Jahng K. Y. ( 2013;). Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 63 16331638 [View Article] [PubMed].
    [Google Scholar]
  17. Kim J.-J., Jin H. M., Lee H. J., Jeon C. O., Kanaya E., Koga Y., Takano K., Kanaya S. ( 2011;). Flavobacterium banpakuense sp. nov., isolated from leaf-and-branch compost. Int J Syst Evol Microbiol 61 15951600 [View Article] [PubMed].
    [Google Scholar]
  18. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  19. Kuo I., Saw J., Kapan D. D., Christensen S., Kaneshiro K. Y., Donachie S. P. ( 2013;). Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 63 32803286 [View Article] [PubMed].
    [Google Scholar]
  20. Lane D. J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematic, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: Willey;.
    [Google Scholar]
  21. Loch T. P., Faisal M. ( 2014;). Flavobacterium spartansii sp. nov., a pathogen of fishes, and emended descriptions of Flavobacterium aquidurense and Flavobacterium araucananum . Int J Syst Evol Microbiol 64 406412 [View Article] [PubMed].
    [Google Scholar]
  22. Marmur J. ( 1961;). A procedure for the isolation of DNA from micro-organisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  23. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [View Article] [PubMed].
    [Google Scholar]
  24. McCammon S. A., Bowman J. P. ( 2000;). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50 10551063 [View Article] [PubMed].
    [Google Scholar]
  25. Miller J. H. ( 1972). Experiments in Molecular Genetics., Cold Spring Harbour, NY: Cold Spring Harbour Laboratory Press;.
    [Google Scholar]
  26. Minnikin D., O'Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  27. Qu J. H., Yuan H. L., Li H. F., Deng C. P. ( 2009;). Flavobacterium cauense sp. nov., isolated from sediment of a eutrophic lake. Int J Syst Evol Microbiol 59 26662669 [View Article] [PubMed].
    [Google Scholar]
  28. Reasoner D. J., Geldreich E. E. ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49 17 [PubMed].
    [Google Scholar]
  29. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  30. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  31. Tamaki H., Hanada S., Kamagata Y., Nakamura K., Nomura N., Nakano K., Matsumura M. ( 2003;). Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53 519526 [View Article] [PubMed].
    [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  33. Van Trappen S., Mergaert J., Swings J. ( 2003;). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53 12411245 [View Article] [PubMed].
    [Google Scholar]
  34. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. ( 2005;). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55 769772 [View Article] [PubMed].
    [Google Scholar]
  35. Wang Z.-W., Liu Y.-H., Dai X., Wang B.-J., Jiang C.-Y., Liu S.-J. ( 2006;). Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56 439442 [View Article] [PubMed].
    [Google Scholar]
  36. Weon H. Y., Song M. H., Son J. A., Kim B. Y., Kwon S. W., Go S. J., Stackebrandt E. ( 2007;). Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 57 15941598 [View Article] [PubMed].
    [Google Scholar]
  37. Yi H., Oh H.-M., Lee J.-H., Kim S.-J., Chun J. ( 2005;). Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55 637641 [View Article] [PubMed].
    [Google Scholar]
  38. Yoon J. H., Kang S. J., Lee J. S., Oh T. K. ( 2007;). Flavobacterium terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 57 947950 [View Article] [PubMed].
    [Google Scholar]
  39. Yoon H. S., Aslam Z., Song G. C., Kim S. W., Jeon C. O., Chon T. S., Chung Y. R. ( 2009;). Flavobacterium sasangense sp. nov., isolated from a wastewater stream polluted with heavy metals. Int J Syst Evol Microbiol 59 11621166 [View Article] [PubMed].
    [Google Scholar]
  40. Zhu F., Wang S., Zhou P. ( 2003;). Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China 1 glacier. Int J Syst Evol Microbiol 53 853857 [View Article] [PubMed].
    [Google Scholar]
  41. Zhu L., Liu Q., Liu H., Zhang J., Dong X., Zhou Y., Xin Y. ( 2013;). Flavobacterium noncentrifugens sp. nov., a psychrotolerant bacterium isolated from glacier meltwater. Int J Syst Evol Microbiol 63 20322037 [View Article] [PubMed].
    [Google Scholar]
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