In this study, we describe strain DCY64T that was isolated from the rhizosphere of three-year-old Korean ginseng root. Cells were Gram-reaction negative, oxidase- and catalase-positive, strictly aerobic, capsulated, non-motile, non-sporulating and spherical to short rod-shaped. Multiplicative budding cells were produced. Vesicles covered the surface of cells. Phylogenetic analysis placed strain DCY64T within the genus Labrys with the highest similarity to Labrys monachus VKM B-1479T (97.6 % 16S rRNA gene sequence similarity), followed by Labrys okinawensis MAFF 210191T (97.5 %), Labrys miyagiensis G24103T (97.4) and Labrys portucalensis F11T (97.0 %). The genomic DNA G+C content was 63 mol%. The presences of summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C19 : 1 cyclo ω8c and C16 : 0 as major fatty acids; phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol as major polar lipids; ubiquinone Q-10 as the predominant quinone and sym-homospermidine as the dominant polyamine were found in strain DCY64T. These chemotaxonomic results were in accordance with those of members of the genus Labrys. However, the absence of C16 : 0 2-OH, C16 : 0 3-OH and C18 : 1 2-OH from the fatty acids profile and differences in minor polar lipids and phenotypic characteristics distinguished strain DCY64T from the closest type strains. The discrimination was also supported by unique enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) fingerprints, as well as DNA–DNA hybridization values ( ≤ 48 %) between strain DCY64T and related type strains. Therefore, we propose that strain DCY64T represents a novel species of the genus Labrys. The name Labrys soli sp. nov. is proposed, with DCY64T ( = KCTC 32173T = JCM 19895T) as the type strain.
AlbertR. A.,
WaasN. E.,
LangerS.,
PavlonsS. C.,
FeldnerJ. L.,
Rosselló-MoraR.,
BusseH. J.
( 2010;). Labrys wisconsinensis sp. nov., a budding bacterium isolated from Lake Michigan water, and emended description of the genus Labrys
. Int J Syst Evol Microbiol601570–1576 [View Article][PubMed].
BarrowG. I.,
FelthamR. K. A.
( 1993). Cowan and Steel's Manual for the Identification of Medical Bacteria
, 3rd edn.., Cambridge:
Cambridge University Press;
[View Article].
CarvalhoM. F.,
De MarcoP.,
DuqueA. F.,
PachecoC. C.,
JanssenD. B.,
CastroP. M. L.
( 2008;). Labrys portucalensis sp. nov., a fluorobenzene-degrading bacterium isolated from an industrially contaminated sediment in northern Portugal. Int J Syst Evol Microbiol58692–698 [View Article][PubMed].
EzakiT.,
HashimotoY.,
YabuuchiE.
( 1989;). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39224–229 [View Article].
FiguerasM. J.,
AlperiA.,
GuarroJ.,
Martínez-MurciaA. J.
( 2006;). Genotyping of isolates included in the description of a novel species should be mandatory. Int J Syst Evol Microbiol561183–1184 [View Article][PubMed].
FritzI.,
StrömplC.,
AbrahamW.-R.
( 2004;). Phylogenetic relationships of the genera Stella, Labrys and Angulomicrobium within the ‘Alphaproteobacteria’ and description of Angulomicrobium amanitiforme sp. nov.. Int J Syst Evol Microbiol54651–657 [View Article][PubMed].
GomoriG.
( 1955;). Preparation of buffers for use in enzyme studies. . In Methods in Enzymology, pp. 138–146. Edited by
ColowickS. P.,
KaplanN. O.
New York:
Academic Press;
[View Article].
HallT. A.
( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser4195–98.
HiraishiA.,
UedaY.,
IshiharaJ.,
MoriT.
( 1996;). Comparative lipoquinone analysis of influent sewage and activated sludge by high performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol42457–469 [View Article].
IslamM. S.,
KawasakiH.,
NakagawaY.,
HattoriT.,
SekiT.
( 2007;). Labrys okinawensis sp. nov. and Labrys miyagiensis sp. nov., budding bacteria isolated from rhizosphere habitats in Japan, and emended descriptions of the genus Labrys and Labrys monachus
. Int J Syst Evol Microbiol57552–557 [View Article][PubMed].
LaneD. J.
( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by
StackebrandtE.,
GoodfellowM.
Chichester:
Willey;.
LarkinM. A.,
BlackshieldsG.,
BrownN. P.,
ChennaR.,
McGettiganP. A.,
McWilliamH.,
ValentinF.,
WallaceI. M.,
WilmA.,
other authors.
( 2007;). clustalwclustal_x version 2.0. Bioinformatics232947–2948 [View Article][PubMed].
MesbahM.,
PremachandranU.,
WhitmanW. B.
( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39159–167 [View Article].
MillerJ. A.,
KalyuzhnayaM. G.,
NoyesE.,
LaraJ. C.,
LidstromM. E.,
ChistoserdovaL.
( 2005;). Labrys methylaminiphilus sp. nov., a novel facultatively methylotrophic bacterium from a freshwater lake sediment. Int J Syst Evol Microbiol551247–1253 [View Article][PubMed].
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.
( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2233–241 [View Article].
MouwenD. J. M.,
WeijtensM. J. B. M.,
CapitaR.,
Alonso-CallejaC.,
PrietoM.
( 2005;). Discrimination of enterobacterial repetitive intergenic consensus PCR types of Campylobacter coli and Campylobacter jejuni by Fourier transform infrared spectroscopy. Appl Environ Microbiol714318–4324 [View Article][PubMed].
SchwynB.,
NeilandsJ. B.
( 1987;). Universal chemical assay for the detection and determination of siderophores. Anal Biochem16047–56 [View Article][PubMed].
StephensonD. P.,
MooreR. J.,
AllisonG. E.
( 2009;). Comparison and utilization of repetitive-element PCR techniques for typing Lactobacillus isolates from the chicken gastrointestinal tract. Appl Environ Microbiol756764–6776 [View Article][PubMed].
TaibiG.,
SchiavoM. R.,
GueliM. C.,
Calanni-RindinaP.,
MuratoreR.,
NicotraC. M. A.
( 2000;). Rapid and simultaneous high-performance liquid chromatography assay of polyamines and monoacetylpolyamines in biological specimens. J Chromatogr B Biomed Sci Appl745431–437 [View Article][PubMed].
TamuraK.,
NeiM.
( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol10512–526[PubMed].
VasilyevaL. V.,
SemenovA. M.
( 1985;). Labrys gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 18. Int J Syst Bacteriol35375–376 [View Article].
VersalovicJ.,
KoeuthT.,
LupskiJ. R.
( 1991;). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res196823–6831 [View Article][PubMed].
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.,
other authors.
( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37463–464 [View Article].