1887

Abstract

A novel actinomycete, strain ST1-08, was isolated from the stem of sp. in Thailand. The taxonomic position of this isolate was determined by using a polyphasic approach. Strain ST1-08 contained -diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate, which are typical properties of members of the genus . Strain ST1-08 grew at 15–40 °C, pH 6–9 and on 5 % (w/v) NaCl. Gelatin liquefaction, starch hydrolysis and skimmed milk peptonization were positive. The strain utilized -arabinose, -glucose, glycerol, -inositol, -mannitol and -rhamnose. The predominant menaquinone was MK-9(H) and the major cellular fatty acids were iso-C and iso-C.The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol. The 16S rRNA gene sequence analysis revealed that the strain was closely related to JCM 12673 (98.99 %) and JCM 12672 (98.87 %). The DNA G+C content of strain ST1-08 was 71.2 mol%. The DNA–DNA relatedness values among strain ST1-08, JCM 12673 and JCM 12672 were lower than 70 %, the cut-off level for assigning strains to the same species. On the basis of phenotypic and genotypic characteristics, strain ST1-08 represents a novel species of the genus , for which the name is proposed. The type strain is ST1-08( = JCM 30050 = PCU 339 = TISTR 2278).

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2015-11-01
2019-10-20
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