1887

Abstract

An extremely halophilic archaeon, strain S2FP14, was isolated from a brine sample from the inland hypersaline lake Fuente de Piedra, a saline-wetland wildfowl reserve located in the province of Málaga in southern Spain. Colonies were red-pigmented and the cells were Gram-staining-negative, motile and pleomorphic. S2FP14 was able to grow in media containing 12.5–30 % (w/v) total salts (optimum 20 %) at pH 7–8.5 (optimum 7.5) and at 25–50 °C (optimum 37 °C). The 16S rRNA gene sequence analysis indicated that this strain represented a member of the genus . S2FP14 showed a similarity of 99.5 % to YC20, 96.1 % to GX31, 95.9 % to TBN53, 95.5 % to YC21, 95.2 % to CBA1103, 94.6 % to CSW2.24.4 and 94.6 % to TNN18. The ′ gene sequence similarity of strain S2FP14 was 97.4 % to 87.6 % with members of genus . The major phospholipids of strain S2FP14 were phosphatidylglycerol phosphate methyl ester and phosphatidylglycerosulfate, plus a very small amount of phosphatidylglycerol and an archaeal analogue of bisphosphatidylglycerol. With regard to glycolipid composition, the most abundant glycolipids were the sulfated diglycosyl diphytanilglyceroldiether and a glycosyl-cardiolipin. The G+C content of strain S2FP14 genomic DNA was 61.4 mol%. The DNA–DNA hybridization between strain S2FP14 and JCM 18361 was 51 %. Based on the phylogenetic, phenotypic and chemotaxonomic features, a novel species, sp. nov. is proposed. The type strain is S2FP14 ( = CECT 8167 = DSM 26177).

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2015-11-01
2019-10-15
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