Phylogenetic and taxonomic characterization was performed for a bacterium, designated strain Q-192T, isolated from the surface of the green macroalga Halimeda sp., collected from the subtropical Ishigaki Island, Japan. The isolate was a polysaccharide-producing, Gram-stain-negative, aerobic, rod-shaped, motile bacterium with a polar flagellum. The isolate was slightly halophilic, required Na+, Mg2+ and Ca2+ ions for growth, but did not require growth factors. The only isoprenoid quinone was ubiquinone-8.The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and C14 : 0. The main hydroxy fatty acid was C10 : 0 3-OH. The DNA G+C content was 45.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolate in the class Gammaproteobacteria. The phylogenetically closest relatives with validly published names were Pseudomaricurvus alkylphenolicus KU41GT, Teredinibacter turnerae T7902T, Pseudoteredinibacter isoporae SW-11T and Simiduia agarivorans SA1T with sequence similarities of 94.5, 94.1, 93.7 and 93.6 %, respectively. The isolate was distinguished from members of these genera by a combination of DNA G+C content, chemotaxonomic characteristics (respiratory quinone system, fatty acid profile and polar lipid composition) and other phenotypic features. Based on phylogenetic, genotypic, chemotaxonomic and phenotypic characteristics, strain Q-192T is considered to represent a novel species of a new genus, for which the name Marinibactrum halimedae gen. nov., sp. nov. is proposed. The type strain of Marinibactrum halimedae is Q-192T ( = NBRC 110095T = NCIMB 14932T).
EkborgN. A.,
GonzálezJ. M.,
HowardM. B.,
TaylorL. E.,
HutchesonS. W.,
WeinerR. M.
( 2005;). Saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. Int J Syst Evol Microbiol551545–1549 [View Article][PubMed].
GonzálezJ. M.,
WeinerR. M.
( 2000;). Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides. Int J Syst Evol Microbiol50831–834 [View Article][PubMed].
IwakiH.,
FujiokaM.,
HasegawaY.
( 2014;). Isolation and characterization of marine nonylphenol-degrading bacteria and description of Pseudomaricurvus alkylphenolicus gen. nov., sp. nov. Curr Microbiol68167–173 [View Article][PubMed].
NakagawaY.,
SakaneT.,
SuzukiM.,
HatanoK.
( 2002;). Phylogenetic structure of the genera Flexibacter, Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis. J Gen Appl Microbiol48155–165 [View Article][PubMed].
NishijimaM.,
Araki-SakaiM.,
SanoH.
( 1997;). Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods28113–122 [View Article].
NishijimaM.,
TakaderaT.,
ImamuraN.,
KasaiH.,
AnK. D.,
AdachiK.,
NagaoT.,
SanoH.,
YamasatoK.
( 2009;). Microbulbifer variabilis sp. nov. and Microbulbifer epialgicus sp. nov., isolated from Pacific marine algae, possess a rod-coccus cell cycle in association with the growth phase. Int J Syst Evol Microbiol591696–1707 [View Article][PubMed].
ParkS.,
KimS.-I.,
JungY.-T.,
YoonJ.-H.
( 2014;). Simiduia curdlanivorans sp. nov., a curdlan-degrading bacterium isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Simiduia
. Int J Syst Evol Microbiol643695–3700 [View Article][PubMed].
ShiehW. Y.,
LiuT. Y.,
LinS. Y.,
JeanW. D.,
ChenJ. S.
( 2008;). Simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. Int J Syst Evol Microbiol58895–900 [View Article][PubMed].
TindallB. J.,
Rosselló-MóraR.,
BusseH.-J.,
LudwigW.,
KämpferP.
( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol60249–266 [View Article][PubMed].
UchinoY.,
YokotaA.,
SugiyamaJ.
( 1997;). Phylogenetic position of the marine subdivision of Agrobacterium species based on 16S rRNA sequence analysis. J Gen Appl Microbiol43243–247 [View Article][PubMed].
UriosL.,
IntertagliaL.,
LesongeurF.,
LebaronP.
( 2011;). Eionea nigra gen. nov., sp. nov., a gammaproteobacterium from the Mediterranean Sea. Int J Syst Evol Microbiol611677–1681 [View Article][PubMed].
YarzaP.,
RichterM.,
PepliesJ.,
EuzébyJ.,
AmannR.,
SchleiferK. H.,
LudwigW.,
GlöcknerF. O.,
Rosselló-MóraR.
( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol31241–250 [View Article][PubMed].
YoshikawaK.,
TakaderaT.,
AdachiK.,
NishijimaM.,
SanoH.
( 1997;). Korormicin, a novel antibiotic specifically active against marine Gram-negative bacteria, produced by a marine bacterium. J Antibiot (Tokyo)50949–953 [View Article][PubMed].