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Abstract

Phylogenetic and taxonomic characterization was performed for a bacterium, designated strain Q-192, isolated from the surface of the green macroalga sp., collected from the subtropical Ishigaki Island, Japan. The isolate was a polysaccharide-producing, Gram-stain-negative, aerobic, rod-shaped, motile bacterium with a polar flagellum. The isolate was slightly halophilic, required Na, Mg and Ca ions for growth, but did not require growth factors. The only isoprenoid quinone was ubiquinone-8.The major cellular fatty acids were Cω7, C and C. The main hydroxy fatty acid was C 3-OH. The DNA G+C content was 45.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolate in the class . The phylogenetically closest relatives with validly published names were KU41G, T7902, SW-11 and SA1 with sequence similarities of 94.5, 94.1, 93.7 and 93.6 %, respectively. The isolate was distinguished from members of these genera by a combination of DNA G+C content, chemotaxonomic characteristics (respiratory quinone system, fatty acid profile and polar lipid composition) and other phenotypic features. Based on phylogenetic, genotypic, chemotaxonomic and phenotypic characteristics, strain Q-192 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is Q-192 ( = NBRC 110095 = NCIMB 14932).

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2015-11-01
2020-01-27
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