%0 Journal Article %A Shimane, Yasuhiro %A Minegishi, Hiroaki %A Echigo, Akinobu %A Kamekura, Masahiro %A Itoh, Takashi %A Ohkuma, Moriya %A Tsubouchi, Taishi %A Usui, Keiko %A Maruyama, Tadashi %A Usami, Ron %A Hatada, Yuji %T Halarchaeum grantii sp. nov., a moderately acidophilic haloarchaeon isolated from a commercial salt sample %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_11 %P 3830-3835 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.000501 %I Microbiology Society, %X Three moderately acidophilic, halophilic archaeal strains, MH1-243-3T, MH1-243-5 and MH1-243-6, were isolated from a commercial salt sample made from seawater in Okinawa, Japan. Cells of the three strains were pleomorphic and stained Gram-negative. Colonies of the strains were orange–red-pigmented. Strain MH1-243-3T was able to grow at 15–27 % (w/v) NaCl (optimum 24 °C), at pH 4.5–6.5 (pH 5.5) and at 35–50 °C (45 °C). Strains MH1-243-5 and MH1-243-6 grew within slightly different ranges (shown in text). The 16S rRNA gene sequences of the three strains were identical, and the closest phylogenetic relative was Halarchaeum salinum MH1-34-1T with 97.0 % similarity. The rpoB′ gene sequences of the three strains were also identical, and the closest phylogenetic relative was Halarchaeum acidiphilum JCM 16109T with 92.0 % similarity. The DNA G+C content of MH1-243-3T, MH1-243-5 and MH1-243-6 was 65.2 mol%. The levels of DNA–DNA relatedness amongst the three strains were 84.1–99.8 %, while that between MH1-243-3T and H. salinum MH1-34-1T was 30.6 % and 31.6 % (reciprocally), and those between MH1-243-3T and type strains of other species in the genus Halarchaeum were 42.3–29.4 %. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolates should represent a novel species of the genus Halarchaeum, for which the name Halarchaeum grantii sp. nov. is proposed. The type strain is MH1-243-3T ( = JCM 19585T = KCTC 4142T), isolated from commercial sea salt produced in Okinawa, Japan. MH1-243-5 ( = JCM 19586) and MH1-243-6 ( = JCM 18422) are additional strains of the species. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000501