sp. nov., isolated from roots of upland rice Free

Abstract

An actinomycete strain, designated CP2R9-1, was isolated from root internal tissues of upland rice (). Based on a polyphasic approach, strain CP2R9-1 was characterized as a member of the genus Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H), MK-9(H) and MK-10(H). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1 was closely related to 232617 (99.32 % similarity), DSM 43168 (99.18 %) and MA-2 (99.16 %). Strain CP2R9-1 was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3 ± 0.1–41.7 ± 0.7 %) and phenotypic differences. The results presented in this study showed that strain CP2R9-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CP2R9-1 ( = BCC 67266 = NBRC 110007).

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2015-11-01
2024-03-29
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