RT Journal Article SR Electronic(1) A1 Kittiwongwattana, Chokchai A1 Thanaboripat, Dusanee A1 Laosinwattana, Chamroon A1 Koohakan, Prommart A1 Parinthawong, Nonglak A1 Thawai, ChittiYR 2015 T1 Micromonospora oryzae sp. nov., isolated from roots of upland rice JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_11 SP 3818 OP 3823 DO https://doi.org/10.1099/ijsem.0.000500 PB Microbiology Society, SN 1466-5034, AB An actinomycete strain, designated CP2R9-1T, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1T was characterized as a member of the genus Micromonospora. meso-Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H4), MK-9(H6) and MK-10(H4). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1T was closely related to Micromonospora haikouensis 232617T (99.32 % similarity), Micromonospora carbonacea DSM 43168T (99.18 %) and Micromonospora krabiensis MA-2T (99.16 %). Strain CP2R9-1T was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3 ± 0.1–41.7 ± 0.7 %) and phenotypic differences. The results presented in this study showed that strain CP2R9-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1T ( = BCC 67266T = NBRC 110007T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000500