1887

Abstract

Two Gram-staining-negative, aerobic, non-motile, red-pigment and rod-shaped bacterial strains, designated RSP19 and RSR28, were isolated from lake water and subjected to polyphasic taxonomic studies. Based on 16S rRNA gene sequence analysis, strains RSP19 and RSR28 exhibited 91.4–95.7 % similarity to the type strains of existing species of the genus . The species most closely related phylogenetically were (95.7 % sequence similarity), (95.4 %), (94.5 %), (94.5 %) and (94.2 %). MK-7 was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and several unidentified polar lipids. The predominant fatty acids were Cω7, iso-C and iso-C 3-OH. The genomic DNA G+C contents were 34.3 and 33.9 mol% for strains RSP19 and RSR28, respectively. Based on the phenotypic characteristics, biochemical properties and genetic analysis, strains RSP19 and RSR28 clearly represent a novel species of the genus , for which the name is proposed. The type strain is RSP19 ( = CGMCC 1.14040 = KCTC 42456 = NBRC 110967).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000498
2015-11-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3782.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000498&mimeType=html&fmt=ahah

References

  1. An D.-S. , Kim S.-G. , Ten L. N. , Cho C.-H. . ( 2009;). Pedobacter daechungensis sp. nov., from freshwater lake sediment in South Korea. Int J Syst Evol Microbiol 59: 69–72.[CrossRef]
    [Google Scholar]
  2. Bernardet J. F. , Nakagawa Y. , Holmes B. . & Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [CrossRef] [PubMed].
    [Google Scholar]
  3. Breznak J. , Costilow R. N. . ( 2007;). Physicochemical factors in growth. . In Methods for General and Molecular Microbiology , 3rd edn., pp. 309–329. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. , Snyder L. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  4. Collins M. D. . ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . London: Academic Press;.
    [Google Scholar]
  5. Collins C. H. , Lyne P. M. , Grange J. M. . ( 1989;). Collins and Lyne's Microbiological Methods London: Butterworth;.
    [Google Scholar]
  6. Derichs J. , Kämpfer P. , Lipski A. . ( 2014;). Pedobacter nutrimenti sp. nov., isolated from chilled food. Int J Syst Evol Microbiol 64: 1310–1316 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum changes for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  9. Hall T. A. . ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
    [Google Scholar]
  10. Kang H. , Kim H. , Joung Y. , Joh K. . ( 2014;). Pedobacter rivuli sp. nov., isolated from a freshwater stream. Int J Syst Evol Microbiol 64: 4073–4078 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kook M. , Park Y. , Yi T. H. . ( 2014;). Pedobacter jejuensis sp. nov., isolated from soil of a pine grove, and emended description of the genus Pedobacter . Int J Syst Evol Microbiol 64: 1789–1794 [CrossRef] [PubMed].
    [Google Scholar]
  13. Lane D. J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M . Chichester: Wiley;.
    [Google Scholar]
  14. Luo X. , Wang Z. , Dai J. , Zhang L. , Li J. , Tang Y. , Wang Y. , Fang C. . ( 2010;). Pedobacter glucosidilyticus sp. nov., isolated from dry riverbed soil. Int J Syst Evol Microbiol 60: 229–233.[CrossRef]
    [Google Scholar]
  15. Margesin R. , Shivaji S . ( 2011;). Genus II. Pedobacter Steyn, Segers, Vancanneyt, Sandra, Kersters and Joubert 1998, 171VP . . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 4, pp. 339–347. Edited by Krieg N. R. , Staley J. T. , Brown D. R. , Hedlund B. P. , Paster B. J. , Ward N. L. , Ludwig W. , Whitman W. B. . New York: Springer;.
    [Google Scholar]
  16. Margesin R. , Spröer C. , Schumann P. , Schinner F. . ( 2003;). Pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite. Int J Syst Evol Microbiol 53: 1291–1296 [CrossRef] [PubMed].
    [Google Scholar]
  17. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  18. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  19. Qiu X. , Qu Z. , Jiang F. , Ren L. , Chang X. , Kan W. , Fang C. , Peng F. . ( 2014;). Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., isolated from Arctic glacier foreland. Int J Syst Evol Microbiol 64: 2431–2436 [CrossRef] [PubMed].
    [Google Scholar]
  20. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  21. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  22. Shivaji S. , Chaturvedi P. , Reddy G. S. N. , Suresh K. . ( 2005;). Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55: 1083–1088 [CrossRef] [PubMed].
    [Google Scholar]
  23. Smibert R. M. , Krieg N. R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  24. Steyn P. L. , Segers P. , Vancanneyt M. , Sandra P. , Kersters K. , Joubert J. J. . ( 1998;). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48: 165–177 [CrossRef] [PubMed].
    [Google Scholar]
  25. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  26. Yoon J. -H. , Kang S. -J. , Park S. , Oh T. -K. . ( 2007;). Pedobacter lentus sp. nov. and Pedobacter terricola sp. nov., isolated from soil. Int J Syst Evol Microbiol 57: 2089–2095.[CrossRef]
    [Google Scholar]
  27. Yumoto I. , Hirota K. , Yamaga S. , Nodasaka Y. , Kawasaki T. , Matsuyama H. , Nakajima K. . ( 2004;). Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54: 1997–2001 [CrossRef] [PubMed].
    [Google Scholar]
  28. Zhou Z. , Jiang F. , Wang S. , Peng F. , Dai J. , Li W. , Fang C. . ( 2012;). Pedobacter arcticus sp. nov., a facultative psychrophile isolated from Arctic soil, and emended descriptions of the genus Pedobacter, Pedobacter heparinus, Pedobacter daechungensis, Pedobacter terricola, Pedobacter glucosidilyticus and Pedobacter lentus . Int J Syst Evol Microbiol 62: 1963–1969 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000498
Loading
/content/journal/ijsem/10.1099/ijsem.0.000498
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error