1887

Abstract

A Gram-staining-positive, coccoid, halotolerant bacterial strain, designated SV-16, was isolated from marine sediment and subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus . Growth occurred at temperatures in the range 25–37 °C (optimum 30 °C), at pH 7.0–11.0 (optimum pH 8.0) and at NaCl concentrations of up to 25.0 % (optimum 15.0 %). The highest level of 16S rRNA gene sequence similarity was with Crm (98.6 %) followed by W24 (96.6 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylglycerol. The major cellular fatty acids were iso-C, anteiso-C, iso-C and anteiso-C. The draft genome of strain SV-16 consisted of 2 591 284 bp with a DNA G+C content of 48.7 mol%. On the basis of the phenotypic characteristics and genotypic distinctiveness of strain SV-16, it should be classified within a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SV-16 ( = MTCC 11832 = DSM 28797).

Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR) (Award grant no. BSC0402)
  • Department of Biotechnology (DBT) Govt. of India (Award grant no. BT/PR7368/INF/22/177/2012)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000495
2015-11-01
2024-11-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3794.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000495&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Schumann P., Hajighasemi M., Ashengroph M., Razavi M. R. ( 2008;). Salinicoccus iranensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 58 178183 [View Article] [PubMed].
    [Google Scholar]
  2. Aslam Z., Lim J. H., Im W. T., Yasir M., Chung Y. R., Lee S. T. ( 2007;). Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57 633638 [View Article] [PubMed].
    [Google Scholar]
  3. Auch A. F., Klenk H.-P., Göker M. ( 2010;). Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2 142148 [View Article] [PubMed].
    [Google Scholar]
  4. Aziz R. K., Bartels D., Best A. A., DeJongh M., Disz T., Edwards R. A., Formsma K., Gerdes S., Glass E. M., other authors. ( 2008;). The rast server: rapid annotations using subsystems technology. BMC Genomics 9 75 [View Article] [PubMed].
    [Google Scholar]
  5. Chen Y. G., Cui X. L., Pukall R., Li H. M., Yang Y. L., Xu L. H., Wen M. L., Peng Q., Jiang C. L. ( 2007;). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57 23272332 [View Article] [PubMed].
    [Google Scholar]
  6. Chen Y. G., Cui X. L., Wang Y. X., Zhang Y. Q., Li Q. Y., Liu Z. X., Wen M. L., Peng Q., Li W. J. ( 2009;). Salinicoccus albus sp. nov., a halophilic bacterium from a salt mine. Int J Syst Evol Microbiol 59 874879 [View Article] [PubMed].
    [Google Scholar]
  7. Claus D., Berkeley R. C. W. ( 1986;). Genus Bacillus Cohn 1872, 174AL . . In Bergey's Manual of Systematic Bacteriology, pp. 11051139. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. vol. 2 Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  8. Collins M. D., Jones D. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diamino butyric acid. J Appl Bacteriol 48 459470 [View Article].
    [Google Scholar]
  9. Cowan S. T., Steel K. J. ( 1965). Manual for the Identification of Medical Bacteria., London: Cambridge University Press;.
    [Google Scholar]
  10. França L., Rainey F. A., Nobre M. F., da Costa M. S. ( 2006;). Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10 531536 [View Article] [PubMed].
    [Google Scholar]
  11. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57 8191 [View Article] [PubMed].
    [Google Scholar]
  12. Jung M. J., Kim M. S., Roh S. W., Shin K. S., Bae J. W. ( 2010;). Salinicoccus carnicancri sp. nov., a halophilic bacterium isolated from a Korean fermented seafood. Int J Syst Evol Microbiol 60 653658 [View Article] [PubMed].
    [Google Scholar]
  13. Kämpfer P., Arun A. B., Busse H. J., Young C. C., Lai W. A., Rekha P. D., Chen W. M. ( 2011;). Salinicoccus sesuvii sp. nov., isolated from the rhizosphere of Sesuvium portulacastrum . Int J Syst Evol Microbiol 61 23482352 [View Article] [PubMed].
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  15. Kim M., Oh H.-S., Park S.-C., Chun J. ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64 346351 [View Article] [PubMed].
    [Google Scholar]
  16. Marquez M. C., Ventosa A., Ruız-Berraquero F. ( 1990;). Marinococcus hispanicus, a new species of moderately halophilic Gram-positive cocci. Int J Syst Bacteriol 40 165169 [View Article].
    [Google Scholar]
  17. Mayilraj S., Saha P., Suresh K., Saini H. S. ( 2006;). Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56 16571661 [View Article] [PubMed].
    [Google Scholar]
  18. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H. P. ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195 413418 [View Article] [PubMed].
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  20. Pakdeeto A., Tanasupawat S., Thawai C., Moonmangmee S., Kudo T., Itoh T. ( 2007;). Salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57 20042008 [View Article] [PubMed].
    [Google Scholar]
  21. Pandey K. K., Mayilraj S., Chakrabarti T. ( 2002;). Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52 15591567 [View Article] [PubMed].
    [Google Scholar]
  22. Qu Z., Li Z., Zhang X., Zhang X. H. ( 2012;). Salinicoccus qingdaonensis sp. nov., isolated from coastal seawater during a bloom of green algae. Int J Syst Evol Microbiol 62 545549 [View Article] [PubMed].
    [Google Scholar]
  23. Ramana C. V., Srinivas A., Subhash Y., Tushar L., Mukherjee T., Kiran P. U., Sasikala C. ( 2013;). Salinicoccus halitifaciens sp. nov., a novel bacterium participating in halite formation. Antonie van Leeuwenhoek 103 885898 [View Article] [PubMed].
    [Google Scholar]
  24. Reddy G. S. N., Matsumoto G. I., Shivaji S. ( 2003;). Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica. Int J Syst Evol Microbiol 53 13631367 [View Article] [PubMed].
    [Google Scholar]
  25. Richter M., Rosselló-Móra R. ( 2009;). Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106 1912619131 [View Article] [PubMed].
    [Google Scholar]
  26. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  27. Schleifer K. H. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18 123156 [View Article].
    [Google Scholar]
  28. Schleifer K. H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477,[PubMed].
    [Google Scholar]
  29. Skerman V. B. D. ( 1967). A Guide to the Identification of the Genera of Bacteria , 2nd edn.., Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  30. Stackebrandt E., Ebers J. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33 152155.
    [Google Scholar]
  31. Stackebrandt E., Goebel B. M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  32. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  33. Tourova T. P., Antonov A. S. ( 1987;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19 333355 [View Article].
    [Google Scholar]
  34. Ventosa A., Marquez M. C., Ruiz-Berraquero F., Kocur M. ( 1990;). Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13 2933 [View Article].
    [Google Scholar]
  35. Ventosa A., Marquez M. C., Weiss N., Tindall B. J. ( 1992;). Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15 530534 [View Article].
    [Google Scholar]
  36. Wang X., Xue Y., Yuan S., Zhou C., Ma Y. ( 2008;). Salinicoccus halodurans sp. nov., a moderate halophile from saline soil in China. Int J Syst Evol Microbiol 58 15371541 [View Article] [PubMed].
    [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology., Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  38. Zhang W., Xue Y., Ma Y., Zhou P., Ventosa A., Grant W. D. ( 2002;). Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer Soda Lake in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 52 789793 [View Article] [PubMed].
    [Google Scholar]
  39. Zhang Y. Q., Yu L. Y., Liu H. Y., Zhang Y. Q., Xu L. H., Li W. J. ( 2007;). Salinicoccus luteus sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 57 19011905 [View Article] [PubMed].
    [Google Scholar]
  40. Zimin A. V., Marçais G., Puiu D., Roberts M., Salzberg S. L., Yorke J. A. ( 2013;). The MaSuRCA genome assembler. Bioinformatics 29 26692677 [View Article] [PubMed].
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.000495
Loading
/content/journal/ijsem/10.1099/ijsem.0.000495
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error