1887

Abstract

Two Gram-stain-negative, rod-shaped bacteria, strains 990B6_12ER2A and 994B6_12ER2A, were isolated during microbiological analysis of a mixed manure sample which was used as input material for a German biogas plant. Phylogenetic identification based on nearly full-length 16S rRNA gene sequences placed the isolates into the family within the phylum . Strains 990B6_12ER2A and 994B6_12ER2A shared identical 16S rRNA gene sequences and showed highest 16S rRNA gene sequence similarity to the type strains of (97.3 %) and (96.8 %).

The major cellular fatty acids of strains 990B6_12ER2A and 994B6_12ER2A were iso-C, summed feature 3 (Cω7 and/or Cω6) and iso-C 3-OH.

The polyamine pattern contained predominantly -homospermidine and the quinone system was menaquinone MK-6. Major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified polar lipid not containing an amino residue, a phosphate residue or a sugar moiety. In addition, moderate to minor amounts of several unidentified lipids were detected. The DNA G+C content was 31.7 and 29.0 mol%, for strains 990B6_12ER2A and 994B6_12ER2A, respectively. On the basis of phylogenetic, chemotaxonomic and physiological analysis we propose a novel species of the genus , sp. nov. (type strain 990B6_12ER2A = CIP 110833 = LMG 28501).

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2015-10-01
2020-09-29
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