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Abstract

A mesophilic, endophytic, filamentous bacterium, designated strain NEAU-gxj18, was isolated from soybean root [ (L.) Merr.] collected from Harbin, Heilongjiang Province, China and characterized using a polyphasic approach. Growth was observed at 20–40 °C (optimum 37 °C). Aerial mycelium was absent on all the media tested. Substrate mycelia were well-developed and formed abundant single endospores with smooth surfaces. The only menaquinone was MK-7.The diagnostic diamino acid was -diaminopimelic acid. The whole-cell sugars were ribose, glucose and galactose. The major fatty acids were iso-C, C and iso-C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid and one undientified phospholipid. The DNA G+C content was 49.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-gxj18 was phylogenetically related to members of the family , with the highest sequence similarity to YIM 77562 (93.35 %). On the basis of morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-gxj18 represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is NEAU-gxj18 ( = CGMCC 4.7223 = DSM 46831).

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2015-10-01
2020-01-23
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