1887

Abstract

A Gram-staining-positive, heterotrophic, anaerobic, non-spore-forming, non-motile, rod-shaped strain, OAct400, belonging to the genus was isolated from a sediment collected from a depth of 2093 m in the South China Sea, China. The strain was identified using a polyphasic taxonomic approach. The strain grew well on yeast extract/malt extract agar (ISP 2) and nutrient agar media, and formed no aerial mycelium and no diffusible pigments on any media tested. The strain grew in the presence of 0–8 % (w/v) NaCl (optimum, 2–4 %), at pH 5.0–10.0 (optimum, pH 7.0) and at 4–37 °C (optimum, 28 °C). Strain OAct400 contained ornithine as the diagnostic diamino acid. The whole-cell sugars were dominated by glucose and galactose. The predominant menaquinones were MK-11 (51 %) and MK-10 (24 %). The major phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C (59.35 %), iso-C (17.89 %) and anteiso-C (16.09 %). DNA–DNA relatedness with DSM 24221 and CIP 107184, the nearest phylogenetic relatives (97.73 and 97.44 % 16S rRNA gene sequence similarity, respectively) was 31.3 ± 2.1 and 28.7 ± 1.2 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, a novel species, sp. nov., is proposed. The type strain is OAct400 ( = CGMCC 4.7181 = DSM 26811 = KCTC 29185).

Funding
This study was supported by the:
  • , the National Natural Science Foundation of China , (Award 31200009)
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2015-10-01
2020-09-22
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