1887

Abstract

A novel autotrophic bacterium, strain skN76, was isolated from sediment of a lake in Japan. As sole electron donor to support chemolithoautotrophic growth, the strain oxidized thiosulfate, tetrathionate and elemental sulfur. For growth, the optimum temperature was 42–45 °C and the optimum pH was 6.8–8.2. The cells were Gram-stain-negative, catalase-positive and oxidase-positive. The strain exhibited changes in morphology depending on growth temperature. Cells grown at the optimum temperature were rod-shaped (0.9–3.0 μm long and 0.3–0.5 μm wide), whereas a filamentous form was observed when the strain was cultured at the lowest permissive growth temperatures. The G+C content of genomic DNA was 69 mol%. The major components in the fatty acid profile were C, summed feature 3 (Cω7 and/or Cω6) and summed feature 9 (iso-Cω9 and/or 10-methyl C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest cultivated relative of strain skN76 was m-1, with sequence similarity of 93 %. On the basis of its phylogenetic and phenotypic properties, strain skN76 ( = DSM 100313 =  NBRC 110942) is proposed as the type strain of a novel species of a novel genus, gen. nov., sp. nov. Novel taxa, fam. nov. and ord. nov., are also proposed to accommodate the genera and gen. nov.

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2015-10-01
2019-12-06
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