1887

Abstract

A novel autotrophic bacterium, strain skN76, was isolated from sediment of a lake in Japan. As sole electron donor to support chemolithoautotrophic growth, the strain oxidized thiosulfate, tetrathionate and elemental sulfur. For growth, the optimum temperature was 42–45 °C and the optimum pH was 6.8–8.2. The cells were Gram-stain-negative, catalase-positive and oxidase-positive. The strain exhibited changes in morphology depending on growth temperature. Cells grown at the optimum temperature were rod-shaped (0.9–3.0 μm long and 0.3–0.5 μm wide), whereas a filamentous form was observed when the strain was cultured at the lowest permissive growth temperatures. The G+C content of genomic DNA was 69 mol%. The major components in the fatty acid profile were C, summed feature 3 (Cω7 and/or Cω6) and summed feature 9 (iso-Cω9 and/or 10-methyl C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest cultivated relative of strain skN76 was m-1, with sequence similarity of 93 %. On the basis of its phylogenetic and phenotypic properties, strain skN76 ( = DSM 100313 =  NBRC 110942) is proposed as the type strain of a novel species of a novel genus, gen. nov., sp. nov. Novel taxa, fam. nov. and ord. nov., are also proposed to accommodate the genera and gen. nov.

Funding
This study was supported by the:
  • Institute for fermentation, Osaka
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000479
2015-10-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3709.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000479&mimeType=html&fmt=ahah

References

  1. Boschker H.T., Vasquez-Cardenas D., Bolhuis H., Moerdijk-Poortvliet T.W., Moodley L. ( 2014;). Chemoautotrophic carbon fixation rates and active bacterial communities in intertidal marine sediments. PLoS One 9 e101443 [View Article] [PubMed].
    [Google Scholar]
  2. Hallberg K.B., Hedrich S., Johnson D.B. ( 2011;). Acidiferrobacter thiooxydans, gen. nov. sp. nov.; an acidophilic, thermo-tolerant, facultatively anaerobic iron- and sulfur-oxidizer of the family Ectothiorhodospiraceae . Extremophiles 15 271279 [View Article] [PubMed].
    [Google Scholar]
  3. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. ( 1984;). Estimation of DNA base composition by high-performance liquid chromatography of its nuclease PI hydrolysate. Agric Biol Chem 48 31693172 [View Article].
    [Google Scholar]
  4. Kelly D.P., Wood A.P. ( 2000;). Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov. Int J Syst Evol Microbiol 50 511516 [View Article] [PubMed].
    [Google Scholar]
  5. Kojima H., Fukui M. ( 2011;). Sulfuritalea hydrogenivorans gen. nov., sp. nov., a facultative autotroph isolated from a freshwater lake. Int J Syst Evol Microbiol 61 16511655 [View Article] [PubMed].
    [Google Scholar]
  6. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  7. Meyer B., Kuever J. ( 2007a;). Molecular analysis of the diversity of sulfate-reducing and sulfur-oxidizing prokaryotes in the environment, using aprA as functional marker gene. Appl Environ Microbiol 73 76647679 [View Article] [PubMed].
    [Google Scholar]
  8. Meyer B., Kuever J. ( 2007b;). Molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5′-phosphosulfate reductase-encoding genes (aprBA) among sulfur-oxidizing prokaryotes. Microbiology 153 34783498 [View Article] [PubMed].
    [Google Scholar]
  9. Oren A. ( 2014;). The family Ectothiorhodospiraceae . . In The Prokaryotes , 4th edn.., pp. 199222. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. Berlin: Springer;.
    [Google Scholar]
  10. Rua C.P.J., Thompson F. ( 2014;). The unclassified genera of Gammaproteobacteria: Alkalimonas, Arenicella, Chromatocurvus, Congregibacter, Gallaecimonas, Halioglobus, Marinicella, Methylohalomonas, Methylonatrum, Orbus, Plasticicumulans, Porticoccus, Sedimenticola, Simiduia, Solimonas . . In The Prokaryotes , 4th edn.., pp. 749768. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. Berlin: Springer; [View Article].
    [Google Scholar]
  11. Selesi D., Schmid M., Hartmann A. ( 2005;). Diversity of green-like and red-like ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbL) in differently managed agricultural soils. Appl Environ Microbiol 71 175184 [View Article] [PubMed].
    [Google Scholar]
  12. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  13. Watanabe M., Kojima H., Fukui M. ( 2014;). Proposal of Effusibacillus lacus gen. nov., sp. nov., and reclassification of Alicyclobacillus pohliae as Effusibacillus pohliae comb. nov. and Alicyclobacillus consociatus as Effusibacillus consociatus comb. nov. Int J Syst Evol Microbiol 64 27702774 [View Article] [PubMed].
    [Google Scholar]
  14. Watanabe T., Kojima H., Fukui M. ( 2015;). Sulfuriferula multivorans gen. nov., sp. nov., isolated from a freshwater lake, reclassification of ‘Thiobacillus plumbophilus’ as Sulfuriferula plumbophilus sp. nov., and description of Sulfuricellaceae fam. nov. and Sulfuricellales ord. nov. Int J Syst Evol Microbiol 65 15041508 [View Article] [PubMed].
    [Google Scholar]
  15. Widdel F., Bak F. ( 1992;). Gram-negative mesophilic sulfate-reducing bacteria. . In The Prokaryotes Vol. 4 , 2nd edn.., pp. 33523378. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer; [View Article].
    [Google Scholar]
  16. Williams K.P., Kelly D.P. ( 2013;). Proposal for a new class within the phylum Proteobacteria, Acidithiobacillia classis nov., with the type order Acidithiobacillales, and emended description of the class Gammaproteobacteria . Int J Syst Evol Microbiol 63 29012906 [View Article] [PubMed].
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.000479
Loading
/content/journal/ijsem/10.1099/ijsem.0.000479
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error