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Abstract

A novel bacterial strain, designated THG-RS2O, was isolated from fallow-land soil previously cultivated with in Yongin, South Korea. Cells were Gram-stain-negative, aerobic, non-motile rods, catalase- and oxidase-positive. Strain THG-RS2O grew optimally at 25–37 °C, at pH 7.0 and in the absence of NaCl. 16S rRNA gene sequence analysis demonstrated that strain THG-RS2O shows highest sequence similarity with KACC 17471 followed by KACC 12505, KACC 12599, KACC 14940 and KCTC 32001. Levels of DNA–DNA relatedness between strain THG-RS2O and the closest phylogenetic neighbours were below 55.0 % and the DNA G+C content of strain THG-RS2O was 63.2 mol%. Major fatty acids were C, cyclo-C and summed feature 3 (Cω6 and/or Cω7). The major respiratory quinone was identified as ubiquonone-8 and predominant polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Characterization by 16S rRNA gene sequence analysis, DNA–DNA hybridization, ubiquinone, polar lipid, fatty acid composition, and physiological and biochemical parameters revealed that strain THG-RS2O represents a novel species of the genus . Hence, the present study describes a novel species for which the name sp. nov. is proposed. The type strain is THG-RS2O ( = KCTC 42609 = CCTCC AB 2015115).

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2015-10-01
2024-03-29
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