1887

Abstract

Thirty-three suspected strains of the family isolated from the oral cavity of polar and brown bears were characterized by genotypic and phenotypic tests. Phylogenetic analysis of partial 16S rRNA gene and sequences showed that the investigated isolates formed two closely related monophyletic groups, representing two novel species of a new genus. Based on 16S rRNA gene sequence comparison was the closest related species with a validly published name, with 95.4 % similarity to the polar bear group and 94.4 % similarity to the brown bear group. was the closest related species based on sequence comparison with a similarity of 89.8 % with the polar bear group and 90 % with the brown bear group. The new genus could be separated from existing genera of the family by three to ten phenotypic characters, and the two novel species could be separated from each other by two phenotypic characters. It is proposed that the strains should be classified as representatives of a new genus, gen. nov., with two novel species: the type species sp. nov., isolated from polar bears (type strain Pb43106 = CCUG 65144 = DSM 28137, DNA G+C content 39.3 mol%), and sp. nov., isolated from brown bears (type strain Bamse61 = CCUG 65145 = DSM 28138).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000476
2015-10-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3683.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000476&mimeType=html&fmt=ahah

References

  1. Abrahamian F.M., Goldstein E.J.C.. ( 2011;). Microbiology of animal bite wound infections. Clin Microbiol Rev 24: 231–246 [CrossRef] [PubMed].
    [Google Scholar]
  2. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J.. ( 1997;). Gapped blast psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402 [CrossRef] [PubMed].
    [Google Scholar]
  3. Angen Ø., Mutters R., Caugant D.A., Olsen J.E., Bisgaard M.. ( 1999;). Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA–DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. Int J Syst Bacteriol 49: 67–86 [CrossRef] [PubMed].
    [Google Scholar]
  4. Angen Ø., Ahrens P., Kuhnert P., Christensen H., Mutters R.. ( 2003a;). Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedisHaemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’. Int J Syst Evol Microbiol 53: 1449–1456 [CrossRef] [PubMed].
    [Google Scholar]
  5. Benson D.A., Karsch-Mizrachi I., Lipman D.J., Ostell J., Wheeler D.L.. ( 2007;). GenBank. Nucleic Acids Res 35: (Database), D21–D25 [CrossRef] [PubMed].
    [Google Scholar]
  6. Birkbeck T.H., Laidler L.A., Grant A.N., Cox D.I.. ( 2002;). Pasteurella skyensis sp. nov., isolated from Atlantic salmon (Salmo salar L.). Int J Syst Evol Microbiol 52: 699–704 [CrossRef] [PubMed].
    [Google Scholar]
  7. Bisgaard M.. ( 1982;). Isolation and characterization of some previously unreported taxa from poultry with phenotypical characters related to Actinobacillus and Pasteurella species. Acta Pathol Microbiol Immunol Scand [B] 90: 59–67.
    [Google Scholar]
  8. Bisgaard M., Houghton S.B., Mutters R., Stenzel A.. ( 1991;). Reclassification of German, British and Dutch isolates of so-called Pasteurella multocida obtained from pneumonic calf lungs. Vet Microbiol 26: 115–124 [CrossRef] [PubMed].
    [Google Scholar]
  9. Bisgaard M., Korczak B.M., Busse H.J., Kuhnert P., Bojesen A.M., Christensen H.. ( 2009;). Classification of the taxon 2 and taxon 3 complex of Bisgaard within Gallibacterium and description of Gallibacterium melopsittaci sp. nov., Gallibacterium trehalosifermentans sp. nov. and Gallibacterium salpingitidis sp. nov. Int J Syst Evol Microbiol 59: 735–744 [CrossRef] [PubMed].
    [Google Scholar]
  10. Blackall P.J., Christensen H., Beckenham T., Blackall L.L., Bisgaard M.. ( 2005;). Reclassification of Pasteurella gallinarum, [Haemophilus, paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov. Int J Syst Evol Microbiol 55: 353–362 [CrossRef] [PubMed].
    [Google Scholar]
  11. Blackall P.J., Bojesen A.M., Christensen H., Bisgaard M.. ( 2007;). Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov. Int J Syst Evol Microbiol 57: 666–674 [CrossRef] [PubMed].
    [Google Scholar]
  12. Christensen H., Bisgaard M.. ( 2004;). Revised definition of Actinobacillus sensu stricto isolated from animals: a review with special emphasis on diagnosis. Vet Microbiol 99: 13–30 [CrossRef] [PubMed].
    [Google Scholar]
  13. Christensen H., Bisgaard M.. ( 2006;). The Genus Pasteurella. . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community Ver. 3.13 Edited by Dworkin M.. etal. New York: Springer;.
    [Google Scholar]
  14. Christensen H., Bisgaard M.. ( 2008;). Taxonomy and biodiversity of members of Pasteurellaceae. . In Pasteurellaceae: Biology, Genomics and Molecular Aspects, pp. 1–26. Edited by Kuhnert P., Christensen H.. Norfolk: Caister Academic Press;.
    [Google Scholar]
  15. Christensen H., Bisgaard M.. ( 2010;). Molecular classification and its impact on diagnostics and understanding the phylogeny and epidemiology of selected members of Pasteurellaceae of veterinary importance. Berl Munch Tierarztl Wochenschr 123: 20–30 [PubMed].
    [Google Scholar]
  16. Christensen H., Bisgaard M., Angen O., Olsen J.E.. ( 2002;). Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii. Int J Syst Evol Microbiol 52: 1239–1246 [CrossRef] [PubMed].
    [Google Scholar]
  17. Christensen H., Bisgaard M., Aalbaek B., Olsen J.E.. ( 2004;). Reclassification of Bisgaard taxon 33, with proposal of Volucribacter psittacicida gen. nov., sp. nov. and Volucribacter amazonae sp. nov. as new members of the Pasteurellaceae. Int J Syst Evol Microbiol 54: 813–818 [CrossRef] [PubMed].
    [Google Scholar]
  18. Christensen H., Kuhnert P., Busse H.-J., Frederiksen W.-C., Bisgaard M.. ( 2007;). Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae. Int J Syst Evol Microbiol 57: 166–178 [CrossRef] [PubMed].
    [Google Scholar]
  19. Christensen H., Korczak B.M., Bojesen A.M., Kuhnert P., Frederiksen W., Bisgaard M.. ( 2011b;). Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group. Int J Syst Evol Microbiol 61: 1829–1836 [CrossRef] [PubMed].
    [Google Scholar]
  20. Christensen H., Kuhnert P., Norskov-Lauritsen N., Planet P.J., Bisgaard M.. ( 2014a;). The Family Pasteurellaceae. . In The Prokaryotes 4th edn, pp. 535–564. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F.. Berlin: Springer;.
    [Google Scholar]
  21. Christensen H., Nicklas W., Bisgaard M.. ( 2014b;). Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov. Int J Syst Evol Microbiol 64: 3636–3643 [CrossRef] [PubMed].
    [Google Scholar]
  22. Eerola E., Lehtonen O.P.. ( 1988;). Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids. J Clin Microbiol 26: 1745–1753 [PubMed].
    [Google Scholar]
  23. Foster G., Ross H.M., Malnick H., Willems A., Hutson R.A., Reid R.J., Collins M.D.. ( 2000;). Phocoenobacter uteri gen. nov., sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from a harbour porpoise (Phocoena phocoena). Int J Syst Evol Microbiol 50: 135–139 [CrossRef] [PubMed].
    [Google Scholar]
  24. Foster G., Higgins R., Leclair D., Korczak B.M., Mikaelian I., Patterson I.A.P., Kuhnert P.. ( 2011;). Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae. Int J Syst Evol Microbiol 61: 3016–3022 [CrossRef] [PubMed].
    [Google Scholar]
  25. Garvie E.I.. ( 1978;). Streptococcus raffinolactis Orla-Jensen and Hansen, a group N streptococcus found in raw milk. Int J Syst Bacteriol 28: 190–193 [CrossRef].
    [Google Scholar]
  26. Gregersen R.H., Neubauer C., Christensen H., Bojesen A.M., Hess M., Bisgaard M.. ( 2009;). Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae. Int J Syst Evol Microbiol 59: 1583–1588 [CrossRef] [PubMed].
    [Google Scholar]
  27. Hansen M.J., Bertelsen M.F., Christensen H., Bojesen A.M., Bisgaard M.. ( 2012;). Otariodibacter oris gen. nov.,sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of pinnipeds. Int J Syst Evol Microbiol 62: 2572–2578 [CrossRef] [PubMed].
    [Google Scholar]
  28. Hansen M.J., Bertelsen M.F., Dietz R., Sonne C., Bojesen A.M.. ( 2013;). A simple and novel method for retrieval of Pasteurellaceae from swab samples collected in the field. MicrobiologyOpen 2: 795–797 [PubMed].
    [Google Scholar]
  29. Hansen M.J., Bertelsen M.F., Christensen H., Bojesen A.M.. ( 2015;). Bisgaardia miroungae sp. nov., a new member of the family Pasteurellaceae isolated from the oral cavity of northern elephant seals (Mirounga angustirostris), and emended description of the genus Bisgaardia. Int J Syst Evol Microbiol 65: 388–392 [CrossRef] [PubMed].
    [Google Scholar]
  30. Korczak B.M., Kuhnert P.. ( 2008;). Phylogeny of Pasteurellaceae. . In Pasteurellaceae: Biology, Genomics and Molecular Aspects, pp. 27–52. Edited by Kuhnert P., Christensen H.. Norfolk: Caister Academic Press;.
    [Google Scholar]
  31. Korczak B., Christensen H., Emler S., Frey J., Kuhnert P.. ( 2004;). Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 54: 1393–1399 [CrossRef] [PubMed].
    [Google Scholar]
  32. Korczak B.M., Bisgaard M., Christensen H., Kuhnert P.. ( 2014;). Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds. Antonie van Leeuwenhoek 105: 731–741 [CrossRef] [PubMed].
    [Google Scholar]
  33. Kuhnert P., Korczak B., Falsen E., Straub R., Hoops A., Boerlin P., Frey J., Mutters R.. ( 2004;). Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease. J Clin Microbiol 42: 5542–5548 [CrossRef] [PubMed].
    [Google Scholar]
  34. Kuhnert P., Scholten E., Haefner S., Mayor D., Frey J.. ( 2010;). Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen. Int J Syst Evol Microbiol 60: 44–50 [CrossRef] [PubMed].
    [Google Scholar]
  35. Lapage S.P., Redway K.F.. ( 1974;). Preservation of Bacteria with Notes on Other Micro-Organisms. Public Health Laboratory Service Monograph Series 7 London: Her Majesty's Stationary Office (HMSO);.
    [Google Scholar]
  36. Mollet C., Drancourt M., Raoult D.. ( 1997;). rpoB sequence analysis as a novel basis for bacterial identification. Mol Microbiol 26: 1005–1011 [CrossRef] [PubMed].
    [Google Scholar]
  37. Montgomery J.M., Gillespie D., Sastrawan P., Fredeking T.M., Stewart G.L.. ( 2002;). Aerobic salivary bacteria in wild and captive Komodo dragons. J Wildl Dis 38: 545–551 [CrossRef] [PubMed].
    [Google Scholar]
  38. Mühldorfer K., Speck S., Wibbelt G.. ( 2014;). Proposal of Vespertiliibacter pulmonis gen. nov., sp. nov. and two genomospecies as new members of the family Pasteurellaceae isolated from European bats. Int J Syst Evol Microbiol 64: 2424–2430 [CrossRef] [PubMed].
    [Google Scholar]
  39. Nørskov-Lauritsen N., Kilian M.. ( 2006;). Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol 56: 2135–2146 [CrossRef] [PubMed].
    [Google Scholar]
  40. Nørskov-Lauritsen N., Bruun B., Kilian M.. ( 2005;). Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov. Int J Syst Evol Microbiol 55: 449–456 [CrossRef] [PubMed].
    [Google Scholar]
  41. Olsen I., Møller K.. ( 2005;). Genus II Actinobacillus Brumpt 1910, 948. . In Bergey's Manual of Systematic Bacteriology, 2nd edn., pp. 866–883. Edited by Garrity G. M., Brenner D. J., Krieg N. R., Staley J. T.. New York: Springer;.
    [Google Scholar]
  42. Olsen G.J., Matsuda H., Hagstrom R., Overbeek R.. ( 1994;). fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10: 41–48 [PubMed].
    [Google Scholar]
  43. Olsen I., Dewhirst F.E., Paster B.J., Busse H.-J.. ( 2005;). Family Pasteurellaceae. . In Bergey's Manual of Systematic Bacteriology, 2nd edn.., pp. 851–856. Edited by Brenner D. J., Krieg N. R., Staley J. T.. New York: Springer;.
    [Google Scholar]
  44. Osawa R., Rainey F., Fujisawa T., Lang E., Busse H.J., Walsh T.P., Stackebrandt E.. ( 1995;). Lonepinella koalarum gen nov, sp nov, a new tannin-protein complex degrading bacterium. Syst Appl Microbiol 18: 368–373 [CrossRef].
    [Google Scholar]
  45. Patel S.M., Mo J.H., Walker M.T., Adley B., Noskin G.A.. ( 2004;). Epidural abscess and osteomyelitis due to Actinobacillus actinomycetemcomitans. Diagn Microbiol Infect Dis 50: 283–285 [CrossRef] [PubMed].
    [Google Scholar]
  46. Rice P., Longden I., Bleasby A.. ( 2000;). emboss: the European molecular biology open software suite. Trends Genet 16: 276–277 [CrossRef] [PubMed].
    [Google Scholar]
  47. Sasser M.. ( 2001;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  48. Snipes K.P., Biberstein E.L.. ( 1982;). Pasteurella testudinis sp. nov.: a parasite of desert tortoises (Gopherus agassizi). Int J Syst Bacteriol 32: 201–210 [CrossRef].
    [Google Scholar]
  49. Stackebrandt, E., Frederiksen, W., Garrity, G.M., Grimont, P.A.D., Kämpfer, P., Maiden, M.C.J., Nesme, X., Rosselló-Mora, R., Swings, J., other authors Stackebrandt E., Frederiksen W., Garrity G.M., Grimont P.A.D., Kämpfer P., Maiden M.C.J., Nesme X., Rosselló-Mora R., Swings J., other authors. ( 2002;). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52: 1043–1047 [CrossRef] [PubMed].
    [Google Scholar]
  50. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  51. Tindall B.J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13: 128–130 [CrossRef].
    [Google Scholar]
  52. Tindall B.J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  53. Viallard V., Poirier I., Cournoyer B., Haurat J., Wiebkin S., Ophel-Keller K., Balandreau J.. ( 1998;). Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia. Int J Syst Bacteriol 48: 549–563 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000476
Loading
/content/journal/ijsem/10.1099/ijsem.0.000476
Loading

Data & Media loading...

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error