1887

Abstract

A Gram-stain-negative, motile, aerobic bacterial strain, designated 22DY15, was isolated from a deep-sea sediment sample collected from a hydrothermal vent field located in the East Pacific Rise. The isolate was a short rod with a single flagellum and was positive for catalase and oxidase activities. Q-10 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphoglycolipid, one aminolipid and three unidentified phospholipids. The principal fatty acid (>70 %) was Cω7. The genomic DNA G+C content was 64.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22DY15 represents a distinct lineage within the family . The closest relatives were species of the genera (93.3–96.0 % 16S rRNA gene sequence similarity), (94.0–96.0 %) and (92.0–95.9 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain 22DY15 could be differentiated from its most closely related genera. Therefore, it is proposed that strain 22DY15 represents a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is 22DY15 ( = JCM 19489 = DSM 27767 = CGMCC 1.12416 = MCCC 1K00276).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000469
2015-10-01
2020-09-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3645.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000469&mimeType=html&fmt=ahah

References

  1. Arahal D.R., Macián M.C., Garay E., Pujalte M.J. ( 2005;). Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 55 23712376 [CrossRef] [PubMed].
    [Google Scholar]
  2. Buchan A., González J.M., Moran M.A. ( 2005;). Overview of the marine roseobacter lineage. Appl Environ Microbiol 71 56655677 [CrossRef] [PubMed].
    [Google Scholar]
  3. Dong X.-Z., Cai M.-Y. ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);.
    [Google Scholar]
  4. Farmer J.J. III, Janda J.M., Brenner F.W., Cameron D.N., Birkhead K.M. ( 2005;). Genus I. Vibrio Pacini 1854, 411AL . . In Bergey's Manual of Systematic Bacteriology, The Proteobacteria, Part B, The Gammaproteobacteria , 2nd edn.. pp. 494546. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;.
    [Google Scholar]
  5. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed].
    [Google Scholar]
  6. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [CrossRef].
    [Google Scholar]
  7. Garrity G.M., Bell J.A., Lilburn T. ( 2005;). Family I. Rhodobacteraceae fam. nov. . In Bergey's Manual of Systematic Bacteriology, 2C , 2nd edn.., p. 161. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;.
    [Google Scholar]
  8. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [CrossRef] [PubMed].
    [Google Scholar]
  10. Kuykendall L.D., Roy M.A., O'Neill J.J., Devine T.E. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38 358361 [CrossRef].
    [Google Scholar]
  11. Leifson E. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85 11831184 [PubMed].
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner,A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32 13631371 [CrossRef] [PubMed].
    [Google Scholar]
  13. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [CrossRef].
    [Google Scholar]
  14. Martínez-Checa F., Quesada E., Martínez-Cánovas M.J., Llamas I., Béjar V. ( 2005;). Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the ‘Alphaproteobacteria’, isolated from a saline soil. Int J Syst Evol Microbiol 55 25252530 [CrossRef] [PubMed].
    [Google Scholar]
  15. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479 297306 [CrossRef] [PubMed].
    [Google Scholar]
  16. Park S., Yoon J.H. ( 2013;). Roseovarius sediminilitoris sp. nov., isolated from seashore sediment. Int J Syst Evol Microbiol 63 17411745 [CrossRef] [PubMed].
    [Google Scholar]
  17. Park Y.D., Baik K.S., Yi H., Bae K.S., Chun J. ( 2005;). Pseudoalteromonas byunsanensis sp. nov., isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 55 25192523 [CrossRef] [PubMed].
    [Google Scholar]
  18. Park S., Park J.M., Kang C.H., Yoon J.H. ( 2015). [CrossRef] [PubMed] Aliiroseovarius pelagivivens gen. nov., sp. nov., isolated from seawater, and reclassification of three Roseovarius species as Aliiroseovarius crassostreae comb. nov., Aliiroseovarius halocynthiae comb. nov. and Aliiroseovarius sediminilitoris comb. nov. Int J Syst Evol Microbiol 65 26462652.
    [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B.M., Ludwig W., Peplies J., Glöckner F.O. ( 2007;). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35 71887196 [CrossRef] [PubMed].
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  21. Stamatakis A. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 26882690 [CrossRef] [PubMed].
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed].
    [Google Scholar]
  23. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 46734680 [CrossRef] [PubMed].
    [Google Scholar]
  24. Tindall B.J., Sikorski J., Smibert R.M., Kreig N.R. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology , 3rd edn.., pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology; [CrossRef].
    [Google Scholar]
  25. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. ( 2007;). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57 16191624 [CrossRef] [PubMed].
    [Google Scholar]
  26. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K.H., Ludwig W., Glöckner F.O., Rosselló-Móra R. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31 241250 [CrossRef] [PubMed].
    [Google Scholar]
  27. Yoon J.H., Kang S.J., Lee S.Y., Oh T.K. ( 2007;). Loktanella maricola sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57 17991802 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000469
Loading
/content/journal/ijsem/10.1099/ijsem.0.000469
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error