1887

Abstract

An aerobic methanotrophic bacterium was isolated from a collapsed palsa soil in northern Norway and designated strain NE2. Cells of this strain were Gram-stain-negative, non-motile, non-pigmented, slightly curved thick rods that multiplied by normal cell division. The cells possessed a particulate methane monooxygenase enzyme (pMMO) and utilized methane and methanol. Strain NE2 grew in a wide pH range of 4.1–8.0 (optimum pH 5.2–6.5) at temperatures between 6 and 32 °C (optimum 18–25 °C), and was capable of atmospheric nitrogen fixation under reduced oxygen tension. The major cellular fatty acids were Cω7, C and Cω7c, and the DNA G+C content was 61.7 mol%. The isolate belonged to the family of the class and was most closely related to the facultative methanotroph KYG (98.3 % 16S rRNA gene sequence similarity and 84 % PmoA sequence identity). However, strain NE2 differed from KYG by cell morphology, the absence of pigmentation, inability to grow on acetate, broader pH growth range, and higher tolerance to NaCl. Therefore, strain NE2 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is NE2 ( = LMG 28715 = VKM B-2945).

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2015-10-01
2019-10-14
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