1887

Abstract

Two independent isolates of a Gram-stain-positive, facultatively anaerobic, motile, rod-shaped bacterium were recovered from cheonggukjang, a Korean fermented soybean paste food product. Preliminary sequencing analysis of the 16S rRNA gene indicated that these strains were related most closely to KCTC-13918 and DSM 13. In phenotypic characterization, the novel strains were found to grow between 15 and 55 °C and to tolerate up to 8 % (w/v) NaCl. Furthermore, the strains grew in media of pH 5–10 (optimal growth at pH 7.0). The predominant cellular fatty acids were anteiso-C and iso-C.The isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown glycolipid. Draft genomes of the two strains were determined and DNA–DNA hybridizations with their nearest neighbour ( KCTC-13918) revealed 29.9 % relatedness for both strains. Phylogenomic analysis of the genomes was conducted with the core genome (799 genes) of all strains in the group and the two strains formed a distinct monophyletic cluster. In addition, the strains differed from the two most closely related species in that they did not metabolize maltose, -galactose, -sorbitol or -gluconic acid. The DNA G+C content was 45.9 mol%. Based upon the consensus of phylogenetic and phenotypic analyses, these strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GO-13 ( = KACC 18425 = NRRL B-65291).

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2015-10-01
2020-01-22
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