1887

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, DSSK2-12, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain DSSK2-12 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DSSK2-12 fell within the clade comprising the type strains of species of the genus . Strain DSSK2-12 exhibited 16S rRNA gene sequence similarity values of 97.2–98.1 % to the type strains of , , and and of 95.39–96.98 % to those of other species of the genus . Strain DSSK2-12 contained Q-8 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), C and Cω7 as the major fatty acids. The major polar lipids of strain DSSK2-12 were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain DSSK2-12 was 48.6 mol% and its mean DNA–DNA relatedness values with the type strains of , , and were 9–21 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain DSSK2-12 is separated from other species of the genus . On the basis of the data presented, strain DSSK2-12 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DSSK2-12 ( = KCTC 42603 = CECT 8870).

Funding
This study was supported by the:
  • , project on survey of indigenous species of Korea of the National Institute of Biological Resources , (Award NRF-2013M3A9A5075953)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000457
2015-10-01
2020-09-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3603.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000457&mimeType=html&fmt=ahah

References

  1. Barrow G.I., Feltham R.K.A., (editors). ( 1993). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press; [CrossRef].
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;.
    [Google Scholar]
  3. Baumann L., Baumann P., Mandel M., Allen R.D. ( 1972;). Taxonomy of aerobic marine eubacteria. J Bacteriol 110 402429. [PubMed].
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51 19972006 [CrossRef] [PubMed].
    [Google Scholar]
  5. Chen Y.-G., Xiao H.-D., Tang S.-K., Zhang Y.-Q., Borrathybay E., Cui X.-L., Li W.-J., Liu Y.-Q. ( 2009;). Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 96 259266 [CrossRef] [PubMed].
    [Google Scholar]
  6. Cohen-Bazire G., Sistrom W.R., Stanier R.Y. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49 2568 [CrossRef] [PubMed].
    [Google Scholar]
  7. Embley T.M., Wait R. ( 1994;). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121161. Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;.
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [CrossRef].
    [Google Scholar]
  9. Ivanova E.P., Ng H.J., Webb H.K., Kurilenko V.V., Zhukova N.V., Mikhailov V.V., Ponamoreva O.N., Crawford R.J. ( 2013;). Alteromonas australica sp. nov., isolated from the Tasman Sea. Antonie van Leeuwenhoek 103 877884 [CrossRef] [PubMed].
    [Google Scholar]
  10. Komagata K., Suzuki K.-I. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161207 [CrossRef].
    [Google Scholar]
  11. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 167 [CrossRef].
    [Google Scholar]
  12. Leifson E. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85 11831184 [PubMed].
    [Google Scholar]
  13. Martínez-Checa F., Béjar V., Llamas I., Del Moral A., Quesada E. ( 2005;). Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 55 23852390 [CrossRef] [PubMed].
    [Google Scholar]
  14. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [CrossRef].
    [Google Scholar]
  15. Park S., Park D.-S., Bae K.S., Yoon J.-H. ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64 13781383 [CrossRef] [PubMed].
    [Google Scholar]
  16. Parte A.C. ( 2014;). LPSN—list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42 (D1), D613D616 [CrossRef] [PubMed].
    [Google Scholar]
  17. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  18. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [CrossRef].
    [Google Scholar]
  19. Staley J.T. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95 19211942 [PubMed].
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [CrossRef].
    [Google Scholar]
  21. Van Trappen S., Tan T.-L., Yang J., Mergaert J., Swings J. ( 2004;). Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas . Int J Syst Evol Microbiol 54 11571163 [CrossRef] [PubMed].
    [Google Scholar]
  22. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., De Vos P., Vandamme P. ( 2008;). Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (approved lists 1980). Int J Syst Evol Microbiol 58 25892596 [CrossRef] [PubMed].
    [Google Scholar]
  23. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [CrossRef].
    [Google Scholar]
  24. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W.Y., Lee S.T., Goodfellow M., Park Y.-H. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46 502505 [CrossRef].
    [Google Scholar]
  25. Yoon J.-H., Lee S.T., Park Y.-H. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48 187194 [CrossRef] [PubMed].
    [Google Scholar]
  26. Yoon J.-H., Kim I.-G., Kang K.H., Oh T.-K., Park Y.-H. ( 2003;). Alteromonas marina sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 53 16251630 [CrossRef] [PubMed].
    [Google Scholar]
  27. Yoon J.-H., Yeo S.-H., Oh T.-K., Park Y.-H. ( 2004;). Alteromonas litorea sp. nov., a slightly halophilic bacterium isolated from an intertidal sediment of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54 11971201 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000457
Loading
/content/journal/ijsem/10.1099/ijsem.0.000457
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error