1887

Abstract

A Gram-staining-negative, halophilic, facultatively anaerobic, motile, rod-shaped and nitrogen-fixing bacterium, designated strain S37, was isolated from an artificial oil-spill sediment sample from the coast of Taean, South Korea. Cells grew at 10–37 °C and pH 5.0–9.0, with optimal growth at 28 °C and pH 6.0–8.0. Growth was observed with 1–9 % (w/v) NaCl in marine broth, with optimal growth with 3–5 % NaCl, but no growth was observed in the absence of NaCl. According to the results of 16S rRNA gene sequence analysis, strain S37 represents a member of the genus of the class and forms a clade with MSSRF60 (97.38 %), ATCC 33466 (97.31 %), ATCC 35048 (97.07 %) J74 (96.76 %) and LMG 13240 (96.76 %). The major fatty acids were C, Cω7/Cω6 and Cω7/Cω6. The DNA G+C content was 41.9 %. The DNA–DNA hybridization analysis results showed a 30.2 % association value with the closely related type strain DSM 21026. On the basis of phenotypic and chemotaxonomic characteristics, strain S37 represents a novel species of the genus , for which the name sp. nov., is proposed with the type strain S37 ( = KEMB 2255-005 = JCM 30409).

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2015-10-01
2019-08-18
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