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Volume 65, Issue Pt_10

sp. nov., isolated from root nodules of R. Br. Free

Abstract

Three novel strains, RITF741, RITF1220 and RITF909, isolated from root nodules of in Guangdong Province of China, have been previously identified as members of the genus , displaying the same 16S rRNA gene RFLP pattern. Phylogenetic analysis of 16S rRNA gene sequences indicated that the three strains belong to the genus and had highest similarity (100.0 %) to LMG 11892. Phylogenetic analyses of housekeeping genes , and revealed that these strains represented a distinct evolutionary lineage within the genus . Strain RITF741 showed >73 % DNA–DNA relatedness with strains RITF1220 and RITF909, but < 60 % DNA–DNA relatedness with the closest type strains of recognized species of the genus . They differed from each other and from their closest phylogenetic neighbours by presence/absence of several fatty acids, or by large differences in the relative amounts of particular fatty acids. While showing distinctive features, they were generally able to utilize a wide range of substrates as sole carbon sources based on API 50CH and API 20NE tests. The three strains were able to form nodules with the original host and other woody legumes such as , and In conclusion, these strains represent a novel species belonging to the genus based on the data obtained in the present and previous studies, for which the name sp. nov. is proposed. The type strain is RITF741 ( = CCBAU 101090 = JCM 30534), the DNA G+C content of which is 64.1 mol% ( ).

  • Published Online:
Funding
This study was supported by the:
  • Special Fund for Forest Scientific Research in the Public Welfare, China (Award 201004075)
  • Fundamental Research Funds for the Central Non-profit Research Institution of RITF (Award RITFYWZX201207)
© 2015 IUMS
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2015-10-01
2024-04-23
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Mesorhizobium acaciae sp. nov., isolated from root nodules of Acacia melanoxylon R. Br.
Int J Syst Evol Microbiol 65, 3558 (2015); https://doi.org/10.1099/ijsem.0.000455
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