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Abstract

Strain KhalAKB1, a mesophilic, anaerobic, rod-shaped bacterium, was isolated from water collected from a mesothermic Tunisian spring. Cells were Gram-staining-positive rods, occurring singly or in pairs and motile by one lateral flagellum. Strain KhalAKB1 grew at 15–45 °C (optimum 30 °C), at pH 5.5–8.5 (optimum pH 7.0) and in the presence of 0–35 g NaCl l (optimum 1 g NaCl l). It fermented yeast extract and a wide range of carbohydrates including cellobiose, -glucose, -ribose, sucrose, -xylose, maltose, -galactose and starch as electron donors. Acetate, ethanol, CO and H were end products of glucose metabolism. It reduced elemental sulfur, but not sulfate, thiosulfate or sulfite, into sulfide. The DNA G+C content was 37.6 mol%. The predominant cellular fatty acids were C and C. Phylogenetic analysis of the 16S rRNA gene sequence suggested as the closest relative of this isolate (identity of 97.2 % to the type strain). Based on phenotypic, phylogenetic and genotypic taxonomic characteristics, strain KhalAKB1 is proposed to be assigned to a novel species within the genus , order , sp. nov. The type strain is KhalAKB1 ( = DSM 28450 = JCM 19912).

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2015-10-01
2021-03-08
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