1887

Abstract

A Gram-staining-negative, non-motile, strictly aerobic bacterium, strain X12M-4, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Strain X12M-4 was catalase- and oxidase-positive. Cells were rod-shaped, 0.5–0.8 μm wide and 1.1–1.6 μm long. Growth was observed in the presence of 0–11.0 % (w/v) NaCl (optimum, 5.0–6.0 %) and at 15–40 °C (optimum, 25 °C) and pH 6.5–9.5 (optimum, pH 7.0). No growth occurred at 10 °C or 45 °C. Strain X12M-4 contained Cω7, C cyclo ω8 and C as the major fatty acids (>10.0 %). The predominant respiratory quinone was Q-10.The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidglycerol, an unknown aminolipid and an unidentified lipid. The DNA G+C content was 65.7 mol% (determined using ). Strain X12M-4 showed highest 16S rRNA gene sequence similarities to HTCC2654 (96.7 %), DSM 19469 (96.4 %), DPG-21 (95.6 %), ‘’ JLT1202r (95.6 %) and M97 (95.5 %) and < 95.5 % to others. Phylogenetic trees based on 16S rRNA gene sequences indicated that strain X12M-4 formed a robust cluster with HTCC2654. On the basis of the data, it is concluded that strain X12M-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is X12M-4 ( = CGMCC 1.12922 = JCM 30555). To accommodate the novel species, the description of the genus was emended.

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2015-10-01
2019-10-20
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