1887

Abstract

A novel bacterial strain, designated Y11, was isolated from marine sediment at Weihai in China. Comparative analysis of 16S rRNA gene sequences demonstrated that the novel isolate showed highest similarity to DSM 9555 (94.0 %) and SS12 (92.7 %). Strain Y11 was a Gram-stain-negative, rod-shaped, non-endospore-forming, yellow-pigmented bacterium and was able to hydrolyse agar weakly. It was catalase-negative, oxidase-positive, facultatively anaerobic and motile by gliding. Optimal growth occurred at 28–30 °C, at pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. The DNA G+C content was 34.4 mol%. The strain contained MK-7 as the prevalent menaquinone. The major cellular fatty acids were iso-C, anteiso-C and Cω6. The predominant polar lipids were phosphatidylethanolamine and two unknown lipids. Data from the present polyphasic taxonomic study clearly place the strain as representing a novel species within the genus , for which the name sp. nov. is proposed. The type strain is Y11 ( = CICC10837 = KCTC42400).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000436
2015-10-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3427.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000436&mimeType=html&fmt=ahah

References

  1. Denger K. , Warthmann R. , Ludwig W. , Schink B. . ( 2002;). Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium. Int J Syst Evol Microbiol 52: 173–178 [PubMed].[CrossRef]
    [Google Scholar]
  2. X. Z Dong , Cai M.Y . . ( 2001;). Manual for the systematic Identification of General Bacteria. , pp. 364–390 Beijing: Science Press;.
    [Google Scholar]
  3. Du Z.-J. , Wang Y. , Dunlap C. , Rooney A.P. , Chen G.-J. . ( 2014;). Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64: 1690–1696 [CrossRef] [PubMed].
    [Google Scholar]
  4. Gao Z.-M. , Liu X. , Zhang X.-Y. , Ruan L.-W. . ( 2013;). Thermophagus xiamenensis gen. nov., sp. nov., a moderately thermophilic and strictly anaerobic bacterium isolated from hot spring sediment. Int J Syst Evol Microbiol 63: 109–113 [CrossRef] [PubMed].
    [Google Scholar]
  5. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y.S. , Lee J.-H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  6. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  7. Kroppenstedt R.M. . ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5: 2359–2367 [CrossRef].
    [Google Scholar]
  8. Liu Q.-Q. , Wang Y. , Li J. , Du Z.-J. , Chen G.-J. . ( 2014;). Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans . Int J Syst Evol Microbiol 64: 2204–2209 [CrossRef] [PubMed].
    [Google Scholar]
  9. Ludwig W. , Euzéby J. , Whitman W.B. , Nakagawa Y. . ( 2011;). Road map of phylum “Bacteroidetes” and Family II. Marinilabiliaceae fam. nov. . In Bergey's Manual of Systematic Bacteriology, Vol. 4, , 2nd edn.., pp. 49–54. Edited by Krieg N. R. , Staley D. R. , Brown B. P. , Hedlund B. J. , Paster N. L. , Ward W. , Ludwig W. B. . New York: Springer;.
    [Google Scholar]
  10. Mesbah M. , Premachandran U. , Whitman W.B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  11. Minnikin D.E. , O'Donnell A.G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J.H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  12. Miyazaki M. , Koide O. , Kobayashi T. , Mori K. , Shimamura S. , Nunoura T. , Imachi H. , Inagaki F. , Nagahama T. , other authors . ( 2012;). Geofilum rubicundum gen. nov., sp. nov., isolated from deep subseafloor sediment. Int J Syst Evol Microbiol 62: 1075–1080 [CrossRef] [PubMed].
    [Google Scholar]
  13. Na H. , Kim S. , Moon E.Y. , Chun J. . ( 2009;). Marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59: 2241–2246 [PubMed].[CrossRef]
    [Google Scholar]
  14. Nakagawa Y. , Yamasato K. . ( 1996;). Emendation of the genus Cytophaga and transfer of Cytophaga agarovorans and Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavobacterium-Cytophaga complex. Int J Syst Bacteriol 46: 599–603 [CrossRef].
    [Google Scholar]
  15. Ruvira M.A. , Lucena T. , Pujalte M.J. , Arahal D.R. , Macián M.C. . et al., ( 2013;). Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal Mediterranean Sea water and emended description of the genus Marinifilum Na et al., 2009. Syst Appl Microbiol 36: 155–159 [CrossRef] [PubMed].
    [Google Scholar]
  16. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  17. Shalley S. , Pradip Kumar S. , Srinivas T.N. , Suresh K. , Anil Kumar P. . ( 2013;). Marinilabilia nitratireducens sp. nov., a lipolytic bacterium of the family Marinilabiliaceae isolated from marine solar saltern. Antonie van Leeuwenhoek 103: 519–525 [CrossRef] [PubMed].
    [Google Scholar]
  18. Sorokin D.Y. , Panteleeva A.N. , Tourova T.P. , Kaparullina E.N. , Muyzer G. . ( 2011;). Natronoflexus pectinivorans gen. nov. sp. nov., an obligately anaerobic and alkaliphilic fermentative member of Bacteroidetes from soda lakes. Extremophiles 15: 691–696 [CrossRef] [PubMed].
    [Google Scholar]
  19. Swofford D.L. . ( 2002;). paup *: Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates;.
  20. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0.. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  21. Thompson J.D. , Gibson T.J. , Plewniak F. , Jeanmougin F. , Higgins D.G. . ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  22. Veldkamp H. . ( 1961;). A study of two marine agar-decomposing, facultatively anaerobic myxobacteria. J Gen Microbiol 26: 331–342 [CrossRef] [PubMed].
    [Google Scholar]
  23. Xiao D. , Zhao F. , Lv M. , Zhang H. , Zhang Y. , Huang H. , Su P. , Zhang Z. , Zhang J. . ( 2012;). Rapid identification of microorganisms isolated from throat swab specimens of community-acquired pneumonia patients by two MALDI-TOF MS systems. Diagn Microbiol Infect Dis 73: 301–307 [CrossRef] [PubMed].
    [Google Scholar]
  24. Yang S.-H. , Seo H.-S. , Woo J.-H. , Oh H.-M. , Jang H. , Lee J.-H. , Kim S.-J. , Kwon K.K. . ( 2014;). Carboxylicivirga gen. nov. in the family Marinilabiliaceae with two novel species Carboxylicivirga mesophila sp. nov. and Carboxylicivirga taeanensis sp. nov., and reclassification of Cytophaga fermentans as Saccharicrinis fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 64: 1351–1358 [CrossRef] [PubMed].
    [Google Scholar]
  25. Zhao B. , Chen S. . ( 2012;). Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake. Int J Syst Evol Microbiol 62: 2618–2623 [CrossRef] [PubMed].
    [Google Scholar]
  26. Zhao C. , Gao Z. , Qin Q. , Ruan L. . ( 2012;). Mangroviflexus xiamenensis gen. nov., sp. nov., a member of the family Marinilabiliaceae isolated from mangrove sediment. Int J Syst Evol Microbiol 62: 1819–1824 [CrossRef] [PubMed].
    [Google Scholar]
  27. Zhilina T.N. , Appel R. , Probian C. , Brossa E.L. , Harder J. , Widdel F. , Zavarzin G.A. . ( 2004;). Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake. Arch Microbiol 182: 244–253 [PubMed].[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000436
Loading
/content/journal/ijsem/10.1099/ijsem.0.000436
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error