1887

Abstract

A Gram-strain-negative, coccoid or oval-shaped, non-motile bacterial strain, designated MDM-1, was isolated from a tidal-flat sediment on the Korean peninsula. Strain MDM-1 was found to grow optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-1 falls within the clade comprising species of the genus , clustering with the type strains of , , and , with which it exhibited 97.2–98.5 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of the other recognized species of the genus were 92.8–97.6 %. Strain MDM-1 was found to contain MK-7 as the predominant menaquinone and iso-C and summed feature 3 (Cω6 and/or Cω7) as the major fatty acids. The major polar lipids were identified as phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain MDM-1 was determined to be 42.7 mol% and the mean DNA–DNA relatedness with KCTC 12051, S1-3, KCTC 23759, DSM 24695 and CIP 107452 was 19.7–5.2 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain MDM-1 is distinguishable from recognized species of the genus . On the basis of the data presented, strain MDM-1 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MDM-1 ( = KCTC 42199 = NBRC 110552).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE)
  • Program for Collection, Management and Utilization of Biological Resources and BK 21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
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2015-10-01
2021-04-15
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