1887

Abstract

In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from and root nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus , and the strains from were most closely related to ACCC 19665 (99.8–99.9 %), IAM 14158 (99.8–99.9 %), USDA 3471 (99.5–99.9 %) and SDW 014 (99.6–99.8 %), whilst the strains from were most closely related to UPM-Ca7 (99.8–99.9 %), CCBAU 33460 (99.7 %) and CCBAU 65327 (99.6 %). Additionally, these strains formed two distinct groups in phylogenetic trees of the housekeeping genes , and . Chemotaxonomic data, including fatty acid profiles, supported the assignment of the strains to the genus and allowed differentiation from the closest neighbours. Results of DNA–DNA hybridizations, MALDI-TOF MS analysis, ERIC-PCR, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their closest neighbouring species. Therefore, the strains isolated from and represent two novel species for which the names sp. nov. (ICMP 19557 = LMG 28228 = HAMBI 3608) and sp. nov. (ICMP 19515 = LMG 28225 = HAMBI 3604), are proposed respectively.

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2015-10-01
2019-10-17
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