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Abstract

An aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, JS14SB-1, was isolated from the surface freshwater of the Jiulong River, PR China. Strain JS14SB-1 grew at 15–38 °C (optimum, 28–35 °C), at pH 6.0–9.0 (optimum pH 7.0) and in the presence of 1.0–7.0 % (w/v) NaCl [optimum 3.0–5.0 % (w/v)]. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain JS14SB-1 was affiliated to the genus , sharing low similarities (95.1–97.1 %) to all type strains of species of this genus. The digital DNA–DNA hybridization (DDH) value between strain JS14SB-1 and the closely related strain SSK3-3 was 20.70 ± 2.33 % and far below the 70 % DDH value taken as the gold standard for delineation of bacterial species. The major fatty acids were identified as iso-C and iso-C 3-OH. The polar lipids were phosphatidylethanolamine, glycolipid, aminolipid, several unidentified phospholipids and lipids. The predominant menaquinone was MK-6. The G+C content of the genomic DNA was 33.8 mol%. Based on the phenotypic, phylogenetic and chemotaxonomic distinctiveness, strain JS14SB-1 is considered to represent a novel species of the genus , for which the name Kordia sp. nov. is proposed; the type strain is JS14SB-1 ( = MCCC 1A00726 = KCTC 42140).

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2015-10-01
2019-09-15
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