%0 Journal Article %A Hezbri, Karima %A Ghodhbane-Gtari, Faten %A Montero-Calasanz, Maria del Carmen %A Sghaier, Haïtham %A Rohde, Manfred %A Schumann, Peter %A Klenk, Hans-Peter %A Gtari, Maher %T Geodermatophilus sabuli sp. nov., a γ-radiation-resistant actinobacterium isolated from desert limestone %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_10 %P 3365-3372 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.000422 %I Microbiology Society, %X A novel γ-radiation-resistant and Gram-staining-positive actinobacterium designated BMG 8133T was isolated from a limestone collected in the Sahara desert of Tunisia. The strain produced dry, pale-pink colonies with an optimum growth at 35–40 °C and pH 6.5–8.0. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid. MK-9(H4) was the dominant menaquinone. Galactose and glucose were detected as diagnostic sugars. The major cellular fatty acids were branched-chain saturated acids iso-C16 : 0 and iso-C15 : 0. The DNA G+C content of the novel strain was 74.5 %. The 16S rRNA gene sequence showed highest sequence identity with Geodermatophilus ruber (98.3 %). Based on phenotypic results and 16S rRNA gene sequence analysis, strain BMG 8133T is proposed to represent a novel species, Geodermatophilus sabuli sp. nov. The type strain is BMG 8133T ( = DSM 46844T = CECT 8820T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000422