1887

Abstract

A novel aerobic actinomycete, designated strain I12A-02601, was isolated from a desert soil crusts sample collected from the Shapotou region of Tengger Desert, north-west China. The substrate mycelia of this isolate were well-developed and branched, but not fragmented. The maturity aerial mycelia formed short chains of small, rod-shaped spores. The strain contained -diaminopimelic acid, -diaminopimelic acid, galactose, glucose, ribose and xylose in its whole-cell hydrolysates. The polar lipids consisted of diphosphatidylglycerol, -acetylglucosamine-containing phospholipids, phosphatidylinositolmannoside and glycolipids. The predominant menaquinones were MK-10(H) and MK-10(H). The major fatty acids were iso-C, anteiso-C, C, anteiso-C and iso-C. The G+C content of the genomic DNA was 72.2 mol%. The 16S rRNA gene sequences comparison showed that strain I12A-02601 was most closely related to members of the family , such as YIM 48868 (93.3 % sequence similarity), PIP 143 (93.2 %), and EUM 378 (93.2 %). In the phylogenetic tree based on 16S rRNA gene sequences, strain I12A-02601 formed a clade with the members of the genera , , and in the family . Combined data from this taxonomic study using a polyphasic approach, led to the conclusion that strain I12A-02601 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is I12A-02601 ( = CPCC 203544 = DSM 45829 = NBRC 109454).

Funding
This study was supported by the:
  • , National Infrastructure of Microbial Resources , (Award NIMR-2014-3)
  • , National Natural Science Foundation of China (NSFC) , (Award 81173026)
  • , National S&T Major Special Project on Major New Drug Innovation , (Award 2012ZX09301002-003)
  • , 863 Program , (Award 2014AA021504)
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2015-10-01
2020-11-30
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