1887

Abstract

A Gram-stain-positive, oxidase- and catalase-negative, rod-shaped, facultatively anaerobic bacterial strain, DCY75, was isolated from a queen wasp (). Growth occurred at 4–37 °C (optimum, 30 °C), at pH 3.5–8.0 (optimum, pH 5.0–6.0) and with ≤ 7.0 % (w/v) NaCl. Strain DCY75 produced gas during growth on glucose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DCY75 belonged to the genus and was closely related to ATCC 27651 and DSM 20690 at sequence similarities of 96.7 and 96.4 %, respectively. A comparison of two housekeeping genes, and , revealed that strain DCT75 was well separated from other species of the genus . Strain DCY75 produced - and -lactic acid isomers in a ratio of 22.5 : 77.5 (v/v). The major fatty acids were summed feature 8 (comprising C ω7 and/or Cω6), C, Cω9 and C.The peptidoglycan structure was of the A4α (-Lys–-Asp) type. Cell-wall sugars were glucose, galactose and ribose. The DNA G+C content was 35.5 ± 1.3 mol%. Based on phenotypic and genotypic properties, strain DCY75 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DCY75 ( = KCTC 21023 = JCM 19742).

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2015-10-01
2019-12-11
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