1887

Abstract

A novel bacterial strain, 29Y89B, was isolated from a faecal sample of a healthy human subject. Cells were Gram-stain-negative, motile, non-spore-forming and rod-shaped. Strain 29Y89B formed cream-coloured colonies 2 mm in diameter on trypticase soy agar and showed optimum growth at 35 °C. Strain 29Y89B showed highest 16S rRNA gene sequence similarity to A18/07 (98.4 %) followed by 1400/07 (97.2 %). Multi-locus sequence analysis using (ATP synthase β subunit), (DNA gyrase), (initiation translation factor 2) and (RNA polymerase β subunit) genes also supported the result of 16S rRNA gene sequence based phylogeny. Strain 29Y89B showed 62 and 40.7 % DNA–DNA relatedness with DSM 22759 and DSM 22758. Strain 29Y89B contained C cyclo, C cyclo ω8, C, C and C as predominant fatty acids. In addition, strain 29Y89B showed physiological and phenotypic differences from its closest relatives DSM 22758 and DSM 22759. The polar lipid profile mainly comprised phospholipids. The DNA G+C content was 59.1 mol%. Thus, based on the findings of the current study, strain 29Y89B showed clear delineations from its closest relatives DSM 22758 and DSM 22759, and is thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 29Y89B ( = DSM 28113 = MCC 2554).

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2015-10-01
2019-09-21
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