1887

Abstract

Three novel endophytic strains, designated 17B10-2-12, 26C10-4-4 and D13-10-4-9, were isolated from the bark of in Heze, Shandong Province, China. They were Gram-reaction-negative, aerobic, non-motile, short-rod-shaped, oxidase-positive and catalase-negative. A phylogenetic analysis of the 16S rRNA gene showed that the three novel strains clustered with members of the family and formed a distinct branch. The isolates shared 100 % similarities among themselves and had the highest sequence similarity with DSM 13620 (95.2 %) and YW8 (95.0 %), and less than 95.0 % sequence similarities with members of other species. Their major fatty acids were C, C cyclo, Cω7 and Cω7/Cω6. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unknown aminophospholipids. The predominant quinone was ubiquinone-8 (Q-8). The DNA G+C content was 69.5–70.0 mol%. Based on data from a polyphasic taxonomy study, the three strains represent a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is 17B10-2-12 ( = CFCC 12099 = KCTC 42091).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200486)
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2015-10-01
2022-01-26
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